About: Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0109875
Description	With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach. With the growing availability of genomic sequence information, there is an increasing need for gene function analysis. Antibody-mediated “silencing” represents an intriguing alternative for the precise inhibition of a particular function of biomolecules. Here, we describe a method for selecting recombinant antibodies with a specific purpose in mind, which is to inhibit intrinsic protein–protein interactions in the cytosol of plant cells. Experimental procedures were designed for conveniently evaluating desired properties of recombinant antibodies in consecutive steps. Our selection method was successfully used to develop a recombinant antibody inhibiting the interaction of ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN 3 with such of its upstream interaction partners as the receiver domain of CYTOKININ INDEPENDENT HISTIDINE KINASE 1. The specific down-regulation of the cytokinin signaling pathway in vivo demonstrates the validity of our approach. (en)
Title	Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies (en)
skos:prefLabel	Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies Targeted In Vivo Inhibition of Specific Protein–Protein Interactions Using Recombinant Antibodies (en)
skos:notation	RIV/00216224:14740/14:00074131!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(EE2.3.20.0189), P(GA13-25280S), P(GA521/09/1699), P(GAP305/11/0756)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Hrdinová, Vendula Zábrady, Matej Hejátko, Jan Janda, Lubomír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	49313
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00074131
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	recombinant antibodies; immunomodulation; inhibition; protein-protein interactions; cytokinin signaling pathway (en)
http://linked.open.../riv/klicoveSlovo	protein-protein interactions immunomodulation inhibition cytokinin signaling pathway recombinant antibodies
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[0ADE81E74929]
http://linked.open...i/riv/nazevZdroje	PLOS ONE : Public Library of Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of excellence in evolutionary cytogenomics, epigenetics and cell signalling Objasnění molekulárních mechanizmů interakce cytokininů a etylénu ve vývoji rostlin Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana Structural basis for the specificity of signal transduction in plants: interaction network of histidine kinase receiver domains in Arabidopsis Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	9
http://linked.open...iv/tvurceVysledku	Hejátko, Jan Janda, Lubomír Zábrady, Matej Hrdinová, Vendula Conrad, Udo Müller, Bruno
http://linked.open...ain/vavai/riv/wos	000343941200081
issn	1932-6203
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0109875
http://localhost/t...ganizacniJednotka	14740

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