About: Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding

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About: Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1093/nar/gku1245
Description	Abasic (AP) lesions are the most frequent type of damages occurring in cellular DNA. Here we describe the conformational effects of AP sites substituted for 2'-deoxyadenosine in the first (ap7), second (ap13) or third (ap19) loop of the quadruplex formed in K+ by the human telomere DNA 5'-d[AG3(TTAG3)3]. CD spectra and electrophoresis reveal that the presence of AP sites does not hinder the formation of intramolecular quadruplexes. NMR spectra show that the structural heterogeneity is substantially reduced in ap7 and ap19 as compared to that in the wild-type. These two (ap7 and ap19) sequences are shown to adopt the hybrid-1 and hybrid-2 quadruplex topology, respectively, with AP site located in a propeller-like loop. All three studied sequences transform easily into parallel quadruplex in dehydrating ethanol solution. Thus, the AP site in any loop region facilitates the formation of the propeller loop. Abasic (AP) lesions are the most frequent type of damages occurring in cellular DNA. Here we describe the conformational effects of AP sites substituted for 2'-deoxyadenosine in the first (ap7), second (ap13) or third (ap19) loop of the quadruplex formed in K+ by the human telomere DNA 5'-d[AG3(TTAG3)3]. CD spectra and electrophoresis reveal that the presence of AP sites does not hinder the formation of intramolecular quadruplexes. NMR spectra show that the structural heterogeneity is substantially reduced in ap7 and ap19 as compared to that in the wild-type. These two (ap7 and ap19) sequences are shown to adopt the hybrid-1 and hybrid-2 quadruplex topology, respectively, with AP site located in a propeller-like loop. All three studied sequences transform easily into parallel quadruplex in dehydrating ethanol solution. Thus, the AP site in any loop region facilitates the formation of the propeller loop. (en)
Title	Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding (en)
skos:prefLabel	Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding Loss of loop adenines alters human telomere d(AG3(TTAG3)3) quadruplex folding (en)
skos:notation	RIV/00216224:14740/14:00073361!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0068), P(EE2.3.20.0042), P(GAP205/12/0466), P(IAA500040903)
http://linked.open...iv/cisloPeriodika	22
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Vorlíčková, Michaela Sklenář, Vladimír Marek, Radek Fiala, Radovan Babinský, Martin Bednářová, Klára Kejnovská, Iva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	26631
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00073361
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Quadruplex DNA; abasic lesion; NMR; CD (en)
http://linked.open.../riv/klicoveSlovo	Quadruplex DNA abasic lesion NMR CD
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[7C467BF48B27]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology DNA quadruplexes in the human genome associated with diseases and aging Biophysics and bioinformatics of genomic DNA fragments very rich in guanine and adenine Internationalisation of the Structural Biology Research Programme with Emphasis on New Directions in R&D
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	42
http://linked.open...iv/tvurceVysledku	Marek, Radek Sklenář, Vladimír Vorlíčková, Michaela Fiala, Radovan Babinský, Martin Bednářová, Klára Kejnovská, Iva Sagi, Janos
http://linked.open...ain/vavai/riv/wos	000347916900057
issn	0305-1048
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1093/nar/gku1245
http://localhost/t...ganizacniJednotka	14740

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