About: The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling

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About: The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12408/epdf
Description	Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacteriumsmegmatis. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) represents a simple reliable approach for rapid bacterial identification based on specific peptide/protein fingerprints. However, cell-wall characteristics of mycobacterial species, and their well known stability, complicate MALDI-TOF MS profiling analysis. In this study, we tested two recently published protocols for inactivation and disruption of mycobacteria, and we also examined the influence of different culture conditions (four culture media and five cultivation times) on mass spectral quality and the discriminatory power of the method. We found a significant influence of sample pretreatment method and culture medium on species identification and differentiation for a total of 10 strains belonging to Mycobacterium phlei and Mycobacteriumsmegmatis. (en)
Title	The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling (en)
skos:prefLabel	The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling The influence of culture conditions on the identification of Mycobacterium species by MALDI-TOF MS profiling (en)
skos:notation	RIV/00216224:14740/14:00073252!RIV15-MSM-14740___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(EE2.3.20.0183), P(QI101A094), S, Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Zdráhal, Zbyněk Šedo, Ondrej Balážová, Tereza
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	21373
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/14:00073252
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MALDI-TOFMS; mycobacteria profiling; strain differentiation; Mycobacterium phlei; Mycobacterium smegmatis (en)
http://linked.open.../riv/klicoveSlovo	MALDI-TOFMS Mycobacterium phlei Mycobacterium smegmatis mycobacteria profiling strain differentiation
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A6A137B21D50]
http://linked.open...i/riv/nazevZdroje	FEMS Microbiology Letters
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Methods of eradication of production diseases of cattle - bovine viral diarrhoea - mucosal disease and paratuberculosis Center of Experimental Biomedicine Central european institute of technology
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	353
http://linked.open...iv/tvurceVysledku	Faldyna, Martin Zdráhal, Zbyněk Šedo, Ondrej Slaný, Michal Makovcová, Jitka Balážová, Tereza
http://linked.open...ain/vavai/riv/wos	000333654500011
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0378-1097
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1111/1574-6968.12408
http://localhost/t...ganizacniJednotka	14740

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