About: Pairing of T-cell receptor chains via emulsion PCR

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Our ability to analyze adaptive immunity and engineer its activity has long been constrained by our limited ability to identify native pairs of heavy-light antibody chains and alpha-beta T-cell receptor (TCR) chains - both of which comprise coupled %22halves of a key%22, collectively capable of recognizing specific antigens. Here we report a cell-based emulsion RT-PCR approach that allows the selective fusion of the native pairs of amplified TCR alpha and beta chain genes for complex samples. A new type of PCR suppression technique was developed that makes it possible to amplify the fused library with minimal noise for subsequent analysis by high-throughput paired-end Illumina sequencing. With this technique, single analysis of a complex blood sample allows identification of multiple native TCR chain pairs. This approach may be extended to identify native antibody chain pairs and, more generally, pairs of mRNA molecules that are co-expressed in the same living cells. Our ability to analyze adaptive immunity and engineer its activity has long been constrained by our limited ability to identify native pairs of heavy-light antibody chains and alpha-beta T-cell receptor (TCR) chains - both of which comprise coupled %22halves of a key%22, collectively capable of recognizing specific antigens. Here we report a cell-based emulsion RT-PCR approach that allows the selective fusion of the native pairs of amplified TCR alpha and beta chain genes for complex samples. A new type of PCR suppression technique was developed that makes it possible to amplify the fused library with minimal noise for subsequent analysis by high-throughput paired-end Illumina sequencing. With this technique, single analysis of a complex blood sample allows identification of multiple native TCR chain pairs. This approach may be extended to identify native antibody chain pairs and, more generally, pairs of mRNA molecules that are co-expressed in the same living cells. (en)
Title	Pairing of T-cell receptor chains via emulsion PCR Pairing of T-cell receptor chains via emulsion PCR (en)
skos:prefLabel	Pairing of T-cell receptor chains via emulsion PCR Pairing of T-cell receptor chains via emulsion PCR (en)
skos:notation	RIV/00216224:14740/13:00068652!RIV14-MSM-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Chudakov, Dmitriy
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	95145
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/13:00068652
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Antibodies; Drug design; discovery; Molecular biology; NGS; TCR (en)
http://linked.open.../riv/klicoveSlovo	Drug design TCR Antibodies discovery NGS Molecular biology
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C5A89D0A75C0]
http://linked.open...i/riv/nazevZdroje	EUROPEAN JOURNAL OF IMMUNOLOGY
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	13 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	43
http://linked.open...iv/tvurceVysledku	Chudakov, Dmitriy Shugay, M. Zvyagin, I. Bolotin, D. Mamedov, I. Putintseva, E. Staroverov, D. Turchaninova, M. Britanova, O. Linnemann, C. Schumacher, T. Sharonov, G. Shcherbo, D.
http://linked.open...ain/vavai/riv/wos	000327819200029
issn	0014-2980
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/eji.201343453
http://localhost/t...ganizacniJednotka	14740

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