About: Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication

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About: Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.ncbi.nlm.nih.gov/pubmed/23419511
Description	A new method for rapid, direct determination of formate in blood serum samples by capillary electrophoresis with contactless conductometric detection is presented. A selective separation of formate was achieved in approximately 1 min using an electrolyte system comprising 10 mM L-histidine, 15 mM glutamic acid and 30 mu M cetyltrimethylammonium bromide at pH 4.56. The only sample preparation was dilution (1:100) with deionized water. The limit of detection and limit of quantitation was 2.2 mu M and 7.3 mu M, respectively, which corresponds to 0.22 mM and 0.73 mM in undiluted blood serum. The method provides a simple and rapid diagnostic test in suspected methanol intoxication cases. The method has been successfully tested on determination of formate in blood of a patient admitted to the hospital under acute methanol intoxication. The peak concentration of formate detected in the patient blood serum was 12.4 mM, which is 10- to 100-fold higher than the normal values in healthy population. A new method for rapid, direct determination of formate in blood serum samples by capillary electrophoresis with contactless conductometric detection is presented. A selective separation of formate was achieved in approximately 1 min using an electrolyte system comprising 10 mM L-histidine, 15 mM glutamic acid and 30 mu M cetyltrimethylammonium bromide at pH 4.56. The only sample preparation was dilution (1:100) with deionized water. The limit of detection and limit of quantitation was 2.2 mu M and 7.3 mu M, respectively, which corresponds to 0.22 mM and 0.73 mM in undiluted blood serum. The method provides a simple and rapid diagnostic test in suspected methanol intoxication cases. The method has been successfully tested on determination of formate in blood of a patient admitted to the hospital under acute methanol intoxication. The peak concentration of formate detected in the patient blood serum was 12.4 mM, which is 10- to 100-fold higher than the normal values in healthy population. (en)
Title	Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication (en)
skos:prefLabel	Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication (en)
skos:notation	RIV/00216224:14740/13:00068155!RIV14-MSM-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068)
http://linked.open...iv/cisloPeriodika	March
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Kubáň, Petr Foret, František
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	64228
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/13:00068155
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Capillary electrophoresis; Contactless conductivity detection; Formate; Methanol intoxication (en)
http://linked.open.../riv/klicoveSlovo	Capillary electrophoresis Contactless conductivity detection Formate Methanol intoxication
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[BC4F915918D4]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	1281
http://linked.open...iv/tvurceVysledku	Foret, František Bocek, Robert Kubáň, Petr
http://linked.open...ain/vavai/riv/wos	000315552700019
issn	0021-9673
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.chroma.2013.01.035
http://localhost/t...ganizacniJednotka	14740

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