About: NMR Determines Transient Structure and Dynamics in the Disordered C-Terminal Domain of WASp Interacting Protein

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About: NMR Determines Transient Structure and Dynamics in the Disordered C-Terminal Domain of WASp Interacting Protein Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.ncbi.nlm.nih.gov/pubmed/23870269
Description	WASp-interacting protein (WIP) is a 503-residue proline-rich polypeptide expressed in human T cells. The WIP C-terminal domain binds to Wiskott-Aldrich syndrome protein (WASp) and regulates its activation and degradation, and the WIP-WASp interaction has been shown to be critical for actin polymerization and implicated in the onset of WAS and X-linked thrombocytopenia. WIP is predicted to be an intrinsically disordered protein, a class of polypeptides that are of great interest because they violate the traditional structure-function paradigm. In this first (to our knowledge) study of WIP in its unbound state, we used NMR to investigate the biophysical behavior of WIPC, a C-terminal domain fragment of WIP that includes residues 407-503 and contains the WASp-binding site. In light of the poor spectral dispersion exhibited by WIPC and the high occurrence (25%) of proline residues, we employed 5D-(NMRC)-C-13-detected NMR experiments with nonuniform sampling to accomplish full resonance assignment. WASp-interacting protein (WIP) is a 503-residue proline-rich polypeptide expressed in human T cells. The WIP C-terminal domain binds to Wiskott-Aldrich syndrome protein (WASp) and regulates its activation and degradation, and the WIP-WASp interaction has been shown to be critical for actin polymerization and implicated in the onset of WAS and X-linked thrombocytopenia. WIP is predicted to be an intrinsically disordered protein, a class of polypeptides that are of great interest because they violate the traditional structure-function paradigm. In this first (to our knowledge) study of WIP in its unbound state, we used NMR to investigate the biophysical behavior of WIPC, a C-terminal domain fragment of WIP that includes residues 407-503 and contains the WASp-binding site. In light of the poor spectral dispersion exhibited by WIPC and the high occurrence (25%) of proline residues, we employed 5D-(NMRC)-C-13-detected NMR experiments with nonuniform sampling to accomplish full resonance assignment. (en)
Title	NMR Determines Transient Structure and Dynamics in the Disordered C-Terminal Domain of WASp Interacting Protein NMR Determines Transient Structure and Dynamics in the Disordered C-Terminal Domain of WASp Interacting Protein (en)
skos:prefLabel	NMR Determines Transient Structure and Dynamics in the Disordered C-Terminal Domain of WASp Interacting Protein NMR Determines Transient Structure and Dynamics in the Disordered C-Terminal Domain of WASp Interacting Protein (en)
skos:notation	RIV/00216224:14740/13:00066376!RIV14-GA0-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GAP206/11/0758)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Žídek, Lukáš Nováček, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	91849
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/13:00066376
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	WISKOTT-ALDRICH-SYNDROME; NUCLEAR-MAGNETIC-RESONANCE; SEQUENCE-SPECIFIC ASSIGNMENT; PROLINE-RICH REGIONS; 8 M UREA; CHEMICAL-SHIFTS; ALPHA-SYNUCLEIN; N-WASP; ACTIN POLYMERIZATION; POLYPROLINE-II (en)
http://linked.open.../riv/klicoveSlovo	8 M UREA ACTIN POLYMERIZATION ALPHA-SYNUCLEIN CHEMICAL-SHIFTS N-WASP POLYPROLINE-II PROLINE-RICH REGIONS SEQUENCE-SPECIFIC ASSIGNMENT WISKOTT-ALDRICH-SYNDROME NUCLEAR-MAGNETIC-RESONANCE
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[8521938259A0]
http://linked.open...i/riv/nazevZdroje	Biophysical Journal
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of high-resolution methodology for NMR studies of disordered proteins with highly degenerated resonance frequencies
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	105
http://linked.open...iv/tvurceVysledku	Nováček, Jiří Žídek, Lukáš Chill, Jordan H Haba, Noam Y Barda-Saad, Mira Gross, Renana Shaked, Hadassa
http://linked.open...ain/vavai/riv/wos	000321941700021
issn	0006-3495
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.bpj.2013.05.046
http://localhost/t...ganizacniJednotka	14740

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