About: Suppression of Peptide Sample Losses in Autosampler Vials

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://pubs.acs.org/doi/pdf/10.1021/pr400183v
Description	Protein or peptide sample losses could accompany all steps of the proteomic analysis workflow. We focused on suppression of sample adsorptive losses during sample storage in autosampler vials. We examined suppression capabilities of six different sample injection solutions and seven types of autosampler vial surfaces using a model sample (tryptic digest of six proteins, 1 fmol per protein). While the vial material did not play an essential role, the choice of appropriate composition of sample injection solution reduced adsorptive losses substantially. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (6 M and 1 M) as injection solutions (both acidified with formic acid (FA) (0.1%)) provided the best results in terms of number of significantly identified peptides (p < 0.05). Protein or peptide sample losses could accompany all steps of the proteomic analysis workflow. We focused on suppression of sample adsorptive losses during sample storage in autosampler vials. We examined suppression capabilities of six different sample injection solutions and seven types of autosampler vial surfaces using a model sample (tryptic digest of six proteins, 1 fmol per protein). While the vial material did not play an essential role, the choice of appropriate composition of sample injection solution reduced adsorptive losses substantially. The combination of a polypropylene vial and solution of poly(ethylene glycol) (PEG) (0.001%) or a mixture of high concentrated urea and thiourea (6 M and 1 M) as injection solutions (both acidified with formic acid (FA) (0.1%)) provided the best results in terms of number of significantly identified peptides (p < 0.05). (en)
Title	Suppression of Peptide Sample Losses in Autosampler Vials Suppression of Peptide Sample Losses in Autosampler Vials (en)
skos:prefLabel	Suppression of Peptide Sample Losses in Autosampler Vials Suppression of Peptide Sample Losses in Autosampler Vials (en)
skos:notation	RIV/00216224:14740/13:00066299!RIV14-MSM-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GBP206/12/G151)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Stejskal, Karel Zdráhal, Zbyněk Potěšil, David
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	109057
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/13:00066299
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	MASS-SPECTROMETRY ANALYSIS; LIQUID-CHROMATOGRAPHY; RECOVERY; PROTEIN; QUANTIFICATION; OPTIMIZATION; PURIFICATION; SURFACTANTS; ADSORPTION; PROTEOMICS (en)
http://linked.open.../riv/klicoveSlovo	PROTEIN MASS-SPECTROMETRY ANALYSIS PROTEOMICS PURIFICATION SURFACTANTS RECOVERY ADSORPTION OPTIMIZATION QUANTIFICATION LIQUID-CHROMATOGRAPHY
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D018E8D7C31C]
http://linked.open...i/riv/nazevZdroje	Journal of Proteome Research
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of novel approaches to bioanalysis and molecular diagnostics Central european institute of technology
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Stejskal, Karel Zdráhal, Zbyněk Potěšil, David
http://linked.open...ain/vavai/riv/wos	000320298600063
issn	1535-3893
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1021/pr400183v
http://localhost/t...ganizacniJednotka	14740

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