About: Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://journals.iucr.org/f/issues/2013/02/00/gj5115/stdsup.html
Description	Histidine-containing phosphotransfer proteins from Arabidopsis thaliana(AHP1–5) act as intermediates between sensor histidine kinases and response regulators in a signalling system called multi-step phosphorelay (MSP). AHP proteins mediate and potentially integrate various MSP-based signalling pathways (e.g. cytokinin or osmosensing). However, structural information about AHP proteins and their importance in MSP signalling is still lacking. To obtain a deeper insight into the structural basis of AHP-mediated signal transduction, the three-dimensional structure of AHP2 was determined. The AHP2 coding sequence was cloned into pRSET B expression vector, enabling production of AHP2 fused to an N-terminal His tag. AHP2 was expressed in soluble form in Escherichia coli strain BL21 (DE3) pLysS and then purified to homogeneity using metal chelate affinity chromatography and anion-exchange chromatography under reducing conditions. Histidine-containing phosphotransfer proteins from Arabidopsis thaliana(AHP1–5) act as intermediates between sensor histidine kinases and response regulators in a signalling system called multi-step phosphorelay (MSP). AHP proteins mediate and potentially integrate various MSP-based signalling pathways (e.g. cytokinin or osmosensing). However, structural information about AHP proteins and their importance in MSP signalling is still lacking. To obtain a deeper insight into the structural basis of AHP-mediated signal transduction, the three-dimensional structure of AHP2 was determined. The AHP2 coding sequence was cloned into pRSET B expression vector, enabling production of AHP2 fused to an N-terminal His tag. AHP2 was expressed in soluble form in Escherichia coli strain BL21 (DE3) pLysS and then purified to homogeneity using metal chelate affinity chromatography and anion-exchange chromatography under reducing conditions. (en)
Title	Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana (en)
skos:prefLabel	Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of AHP2, a signal transmitter protein from Arabidopsis thaliana (en)
skos:notation	RIV/00216224:14740/13:00066056!RIV14-MSM-14740___
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0068), P(ED2.1.00/01.0024), P(GA521/09/1699), P(GAP305/11/0756)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Hejátko, Jan Janda, Lubomír Dopitová, Radka Nejedlá, Eliška
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	65718
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/13:00066056
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	multistep phosphorelay; AHP2; thermal shift assay; crystallization; vapor diffusion method; X-ray diffraction (en)
http://linked.open.../riv/klicoveSlovo	multistep phosphorelay X-ray diffraction crystallization AHP2 thermal shift assay vapor diffusion method
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[FEE29FE5560D]
http://linked.open...i/riv/nazevZdroje	Acta Crystalographica Section F
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Immunomodulation as a functional proteomics tool for cytokinin signaling study in Arabidopsis thaliana Structural basis for the specificity of signal transduction in plants: interaction network of histidine kinase receiver domains in Arabidopsis Central european institute of technology South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	69
http://linked.open...iv/tvurceVysledku	Dopitová, Radka Hejátko, Jan Janda, Lubomír Kutá-Smatanová, Ivana Kutý, Michal Nejedlá, Eliška Degtjarik, Oksana Puehringer, Sandra Weiss, Manfred
http://linked.open...ain/vavai/riv/wos	000314467200017
issn	1744-3091
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1107/S174430911205186X
http://localhost/t...ganizacniJednotka	14740

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