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Description
  • Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH.
  • Chromosome painting (CP) refers to visualization of large chromosome regions, entire chromosome arms, or entire chromosomes via fluorescence in situ hybridization (FISH). For CP in plants, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the target species or from a closely related species (comparative chromosome painting, CCP) are typically applied as painting probes. Extended pachytene chromosomes provide the highest resolution of CP in plants. CP enables identification and tracing of particular chromosome regions and/or entire chromosomes throughout all meiotic stages as well as corresponding chromosome territories in premeiotic interphase nuclei. Meiotic pairing and structural chromosome rearrangements (typically inversions and translocations) can be identified by CP. Here, we describe step-by-step protocols of CP and CCP in plant species including chromosome preparation, BAC DNA labeling, and multicolor FISH. (en)
Title
  • Analysis of Plant Meiotic Chromosomes by Chromosome Painting
  • Analysis of Plant Meiotic Chromosomes by Chromosome Painting (en)
skos:prefLabel
  • Analysis of Plant Meiotic Chromosomes by Chromosome Painting
  • Analysis of Plant Meiotic Chromosomes by Chromosome Painting (en)
skos:notation
  • RIV/00216224:14740/13:00065831!RIV14-GA0-14740___
http://linked.open...avai/riv/aktivita
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  • P(GAP501/10/1014), P(IAA601630902)
http://linked.open...vai/riv/dodaniDat
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  • 60606
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  • RIV/00216224:14740/13:00065831
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  • Chromosome painting; Fluorescence in situ hybridization; BAC FISH; Pachytene chromosomes; DNA labeling (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [E9277229B59D]
http://linked.open...i/riv/mistoVydani
  • New York
http://linked.open...vEdiceCisloSvazku
  • Part 1
http://linked.open...i/riv/nazevZdroje
  • Plant Meiosis: Methods and Protocols
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  • Lysák, Martin
  • Mandáková, Terezie
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/978-1-62703-333-6_2
http://purl.org/ne...btex#hasPublisher
  • Humana Press
https://schema.org/isbn
  • 9781627033329
http://localhost/t...ganizacniJednotka
  • 14740
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