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Description
  • Application of Tris-N-[Tris(hydroxymethyl)methyl]glycine gels for 2DE is hampered by formation of mixed CHAPS-SDS micelles resulting in typical swirling pattern in the low mass range, which makes reliable quantitative and qualitative gel evaluation impossible. Modification of 2DE strip equilibration procedure prevented the direct interaction between both detergents during equilibration process, thus substantially improving gel separation.
  • Application of Tris-N-[Tris(hydroxymethyl)methyl]glycine gels for 2DE is hampered by formation of mixed CHAPS-SDS micelles resulting in typical swirling pattern in the low mass range, which makes reliable quantitative and qualitative gel evaluation impossible. Modification of 2DE strip equilibration procedure prevented the direct interaction between both detergents during equilibration process, thus substantially improving gel separation. (en)
Title
  • Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels
  • Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels (en)
skos:prefLabel
  • Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels
  • Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels (en)
skos:notation
  • RIV/00216224:14740/13:00065684!RIV14-MZE-14740___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED1.1.00/02.0068), P(GBP206/12/G151), P(QI91A069)
http://linked.open...iv/cisloPeriodika
  • 13
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 99150
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14740/13:00065684
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • 2DE; Mixed detergent micelles; Tris-Tricine gel (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [115491BFF426]
http://linked.open...i/riv/nazevZdroje
  • Electrophoresis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 34
http://linked.open...iv/tvurceVysledku
  • Bárta, Jan
  • Konečná, Hana
  • Zdráhal, Zbyněk
  • Lochmanová, Gabriela
  • Fridrichová, Danuše
http://linked.open...ain/vavai/riv/wos
  • 000322112200017
issn
  • 0173-0835
number of pages
http://bibframe.org/vocab/doi
  • 10.1002/elps.201300084
http://localhost/t...ganizacniJednotka
  • 14740
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