About: Identification of cytokinin receptors of Brassica napus

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	https://vufind.mzk.cz/Record/MZK01-001228453/UserComments
Description	Cytokinins (CK) belong to a group of plant hormones, playing crucial role in plant growth and development. CK signal is transduced through a multistep phosphorelay where the phosphoryl group is transferred between His and Asp residues. Cytokinin perception has been well-studied and the CHASE-containing sensor His kinases (CHKs) have been shown to be important morphological factors in Arabidopsis. As Brassica napus shares a high degree of nucleotide identity in coding regions with Arabidopsis, we used sequences of A. thaliana His kinase genes (AHKs) to search for their orthologs in B. napus (BnCHKs). Experimental screen of bacterial artificial chromosome (BAC) genomic DNA library of B. napus, var. Tapidor (JBnB), resulted in identification of redundant number of overlapping BAC clones, which hybridized with PCR-generated probes derived from sequences of phylogenetically conserved AHK regions. Five selected clones have been sequenced and defined as CK receptors by computational analysis. Cytokinins (CK) belong to a group of plant hormones, playing crucial role in plant growth and development. CK signal is transduced through a multistep phosphorelay where the phosphoryl group is transferred between His and Asp residues. Cytokinin perception has been well-studied and the CHASE-containing sensor His kinases (CHKs) have been shown to be important morphological factors in Arabidopsis. As Brassica napus shares a high degree of nucleotide identity in coding regions with Arabidopsis, we used sequences of A. thaliana His kinase genes (AHKs) to search for their orthologs in B. napus (BnCHKs). Experimental screen of bacterial artificial chromosome (BAC) genomic DNA library of B. napus, var. Tapidor (JBnB), resulted in identification of redundant number of overlapping BAC clones, which hybridized with PCR-generated probes derived from sequences of phylogenetically conserved AHK regions. Five selected clones have been sequenced and defined as CK receptors by computational analysis. (en)
Title	Identification of cytokinin receptors of Brassica napus Identification of cytokinin receptors of Brassica napus (en)
skos:prefLabel	Identification of cytokinin receptors of Brassica napus Identification of cytokinin receptors of Brassica napus (en)
skos:notation	RIV/00216224:14740/12:00064588!RIV13-MSM-14740___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(LC06034), S, Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Hejátko, Jan Kuderová, Alena Šmajs, David Nejedlá, Eliška
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	140285
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/12:00064588
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	plant hormone- multistep His-Asp phosphorelay-cytokinin receptor-CHASE-containing histidine kinase-Arabidopsis-Brassica napus-JBnB library-heterologous hybridization-gene structure-BnCHK (en)
http://linked.open.../riv/klicoveSlovo	plant hormone- multistep His-Asp phosphorelay-cytokinin receptor-CHASE-containing histidine kinase-Arabidopsis-Brassica napus-JBnB library-heterologous hybridization-gene structure-BnCHK
http://linked.open...ontrolniKodProRIV	[0B4A10D0E8DB]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Regulation of morphogenesis of plant cells and organs
http://linked.open...UplatneniVysledku	2012
http://linked.open...iv/tvurceVysledku	Hejátko, Jan Micenková, Lenka Šmajs, David Kuderová, Alena Nejedlá, Eliška Kuricová, Katarína Čurdová, Anna
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14740

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