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Description
| - We focused on the possibility of determination green fluorescent protein (GFP) and Arabidopsis histidine phophotransfer protein 5 fused with GFP (AHP5-GFP) in complex plant samples using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The control method is western blotting, where the anti-GFP antibodies enable monitoring the expression level of AHP5-GFP in individual organisms. It was found that CE-LIF is sensitive enough for both proteins. In the future, the developed methods should be used to analyze the phosphorylated and unphosphorylated AHP5-GFP.
- We focused on the possibility of determination green fluorescent protein (GFP) and Arabidopsis histidine phophotransfer protein 5 fused with GFP (AHP5-GFP) in complex plant samples using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The control method is western blotting, where the anti-GFP antibodies enable monitoring the expression level of AHP5-GFP in individual organisms. It was found that CE-LIF is sensitive enough for both proteins. In the future, the developed methods should be used to analyze the phosphorylated and unphosphorylated AHP5-GFP. (en)
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Title
| - Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF
- Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF (en)
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skos:prefLabel
| - Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF
- Analysis of GFP and AHP5-GFP Fusion Protein in the Plant Extract Using CE-LIF (en)
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skos:notation
| - RIV/00216224:14740/12:00057866!RIV13-GA0-14740___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(ED1.1.00/02.0068), P(GA203/09/1025), P(GAP206/12/0538), S, Z(MSM0021622415)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14740/12:00057866
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - CE-LIF; GF; fusion protein AHP5-GFP (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - CECE 2012 9th International Interdisciplinary Meeting on Bioanalysis
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Dopitová, Radka
- Hejátko, Jan
- Michalcová, Lenka
- Preisler, Jan
- Válková, Martina
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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http://linked.open...n/vavai/riv/zamer
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - 9th International Interdisciplinary Meeting on Bioanalysis, CECE 2012
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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