About: Stacking Interactions between Carbohydrate and Protein Quantified by Combination of Theoretical and Experimental Methods

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About: Stacking Interactions between Carbohydrate and Protein Quantified by Combination of Theoretical and Experimental Methods Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0046032
Description	Carbohydrate - receptor interactions are an integral part of biological events. They play an important role in many cellular processes, such as cell-cell adhesion, cell differentiation and in-cell signaling. Carbohydrates can interact with a receptor by using several types of intermolecular interactions. One of the most important is the interaction of a carbohydrate's apolar part with aromatic amino acid residues, known as dispersion interaction or CH/pi interaction. In the study presented here, we attempted for the first time to quantify how the CH/pi interaction contributes to a more general carbohydrate - protein interaction. We used a combined experimental approach, creating single and double point mutants with high level computational methods, and applied both to Ralstonia solanacearum (RSL) lectin complexes with alpha-L-Me-fucoside. Experimentally measured binding affinities were compared with computed carbohydrate-aromatic amino acid residue interaction energies. Carbohydrate - receptor interactions are an integral part of biological events. They play an important role in many cellular processes, such as cell-cell adhesion, cell differentiation and in-cell signaling. Carbohydrates can interact with a receptor by using several types of intermolecular interactions. One of the most important is the interaction of a carbohydrate's apolar part with aromatic amino acid residues, known as dispersion interaction or CH/pi interaction. In the study presented here, we attempted for the first time to quantify how the CH/pi interaction contributes to a more general carbohydrate - protein interaction. We used a combined experimental approach, creating single and double point mutants with high level computational methods, and applied both to Ralstonia solanacearum (RSL) lectin complexes with alpha-L-Me-fucoside. Experimentally measured binding affinities were compared with computed carbohydrate-aromatic amino acid residue interaction energies. (en)
Title	Stacking Interactions between Carbohydrate and Protein Quantified by Combination of Theoretical and Experimental Methods Stacking Interactions between Carbohydrate and Protein Quantified by Combination of Theoretical and Experimental Methods (en)
skos:prefLabel	Stacking Interactions between Carbohydrate and Protein Quantified by Combination of Theoretical and Experimental Methods Stacking Interactions between Carbohydrate and Protein Quantified by Combination of Theoretical and Experimental Methods (en)
skos:notation	RIV/00216224:14740/12:00057813!RIV13-GA0-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GA303/09/1168), P(GD301/09/H004)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kozmon, Stanislav Komárek, Jan Koča, Jaroslav Wimmerová, Michaela Mishra, Sushil Kumar Nečasová, Ivona
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	170870
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/12:00057813
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	SMALL MOLECULAR-INTERACTIONS; DENSITY-FUNCTIONAL THEORY; SEPARATE TOTAL ENERGIES; INTERACTIONS AB-INITIO; DOT-PI-INTERACTIONS; O HYDROGEN-BONDS; AROMATIC INTERACTIONS; RALSTONIA-SOLANACEARUM; STRUCTURAL BASIS; EXCHANGE-ENERGY (en)
http://linked.open.../riv/klicoveSlovo	DENSITY-FUNCTIONAL THEORY AROMATIC INTERACTIONS DOT-PI-INTERACTIONS EXCHANGE-ENERGY INTERACTIONS AB-INITIO O HYDROGEN-BONDS RALSTONIA-SOLANACEARUM SEPARATE TOTAL ENERGIES SMALL MOLECULAR-INTERACTIONS STRUCTURAL BASIS
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D63D110C373B]
http://linked.open...i/riv/nazevZdroje	Plos One
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Lectins from human pathogens - structure, function, engineering Molecular and structural biology of selected antitumor drugs. From mechanistic studies to chemotherapy of tumors
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Koča, Jaroslav Komárek, Jan Kozmon, Stanislav Wimmerová, Michaela Mishra, Sushil Kumar Nečasová, Ivona
http://linked.open...ain/vavai/riv/wos	000309831500025
issn	1932-6203
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0046032
http://localhost/t...ganizacniJednotka	14740

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