About: In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination

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About: In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.nature.com/emboj/journal/v31/n19/abs/emboj2012237a.html
Description	The Nrd1-Nab3-Sen1 (NNS) complex pathway is responsible for transcription termination of cryptic unstable transcripts and sn/snoRNAs. The NNS complex recognizes short motifs on the nascent RNA, but the presence of these sequences alone is not sufficient to define a functional terminator. We generated a homogeneous set of several hundreds of artificial, NNS-dependent terminators with an in vivo selection approach. Analysis of these terminators revealed novel and extended sequence determinants for transcription termination and NNS complex binding as well as supermotifs that are critical for termination. Biochemical and structural data revealed that affinity and specificity of RNA recognition by Nab3p relies on induced fit recognition implicating an a-helical extension of the RNA recognition motif. The Nrd1-Nab3-Sen1 (NNS) complex pathway is responsible for transcription termination of cryptic unstable transcripts and sn/snoRNAs. The NNS complex recognizes short motifs on the nascent RNA, but the presence of these sequences alone is not sufficient to define a functional terminator. We generated a homogeneous set of several hundreds of artificial, NNS-dependent terminators with an in vivo selection approach. Analysis of these terminators revealed novel and extended sequence determinants for transcription termination and NNS complex binding as well as supermotifs that are critical for termination. Biochemical and structural data revealed that affinity and specificity of RNA recognition by Nab3p relies on induced fit recognition implicating an a-helical extension of the RNA recognition motif. (en)
Title	In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination (en)
skos:prefLabel	In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1 dependent transcription termination (en)
skos:notation	RIV/00216224:14740/12:00057509!RIV13-GA0-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GAP305/10/1490), P(GBP305/12/G034)
http://linked.open...iv/cisloPeriodika	19
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Kubíček, Karel Štefl, Richard Hóbor, Fruzsina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	141054
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/12:00057509
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cryptic unstable transcripts; transcription termination; Nrd1p-Nab3p-Sen1p complex; hidden transcription; transcriptional insulators (en)
http://linked.open.../riv/klicoveSlovo	transcription termination Nrd1p-Nab3p-Sen1p complex cryptic unstable transcripts hidden transcription transcriptional insulators
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[7FB9D430ED1C]
http://linked.open...i/riv/nazevZdroje	EMBO Journal
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre for RNA Biology Central european institute of technology Structural basis for poly(A) independent transcription termination pathway
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Kubíček, Karel Hóbor, Fruzsina Štefl, Richard Boulay, Jocelyne D’Aubenton-Carafa, Yves Gudipati, Rajani Kanth Libri, Domenico Porrua-Fuerte, Odil
http://linked.open...ain/vavai/riv/wos	000309656600015
issn	0261-4189
number of pages	14 (xsd:int)
http://bibframe.org/vocab/doi	10.1038/emboj.2012.237
http://localhost/t...ganizacniJednotka	14740

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