About: Expression of glycosyltransferase in fussion with MBP and following TEV protease cleavage

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About: Expression of glycosyltransferase in fussion with MBP and following TEV protease cleavage Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Members of the genus Mycobacterium are responsible for major health problems in humans. Mycobacterium tuberculosis is gram positive, acid resistent bacterium causing chronic illness. Due to the essentiality of mycobacterial cell wall, the enzymes responsible for its synthesis pose a wellcome target for design of new therapeutics. Rv3782 is galactofuranosyl transferase responsible for attaching the first and, perhaps, the second Galf unit to the C50-P-P-GlcNAc-Rha acceptor in the biosynthetic pathway of arabinogalactan. Glycosyltransferases are in general notoriously difficult to express and purify. Several expression-purification strategies have been applied on this enzyme without satisfying outcome. Modified scheme for purification of Rv3782 for structure-functional studies will be presented. Expression of MBP, TEV cleavage site and Rv3782 in one polypeptide chain faciliated by recombinant DNA techniques could lead to successfull purification of Rv3782. Members of the genus Mycobacterium are responsible for major health problems in humans. Mycobacterium tuberculosis is gram positive, acid resistent bacterium causing chronic illness. Due to the essentiality of mycobacterial cell wall, the enzymes responsible for its synthesis pose a wellcome target for design of new therapeutics. Rv3782 is galactofuranosyl transferase responsible for attaching the first and, perhaps, the second Galf unit to the C50-P-P-GlcNAc-Rha acceptor in the biosynthetic pathway of arabinogalactan. Glycosyltransferases are in general notoriously difficult to express and purify. Several expression-purification strategies have been applied on this enzyme without satisfying outcome. Modified scheme for purification of Rv3782 for structure-functional studies will be presented. Expression of MBP, TEV cleavage site and Rv3782 in one polypeptide chain faciliated by recombinant DNA techniques could lead to successfull purification of Rv3782. (en)
Title	Expression of glycosyltransferase in fussion with MBP and following TEV protease cleavage Expression of glycosyltransferase in fussion with MBP and following TEV protease cleavage (en)
skos:prefLabel	Expression of glycosyltransferase in fussion with MBP and following TEV protease cleavage Expression of glycosyltransferase in fussion with MBP and following TEV protease cleavage (en)
skos:notation	RIV/00216224:14740/11:00053914!RIV12-MSM-14740___
http://linked.open...avai/riv/aktivita	S Z
http://linked.open...avai/riv/aktivity	S, Z(MSM0021622413)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kostlánová, Nikola Wimmerová, Michaela Kyseľ, Peter Rotková, Gabriela
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	199022
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00053914
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	arabinogalactan; cell wall; galactofuranosyl transferase; glycosyltransferase; maltose binding protein; mycobacterium; protease cleavage; purification; TEV protease; tuberculosis (en)
http://linked.open.../riv/klicoveSlovo	tuberculosis TEV protease arabinogalactan galactofuranosyl transferase glycosyltransferase maltose binding protein mycobacterium protease cleavage cell wall purification
http://linked.open...ontrolniKodProRIV	[D2A9C1A7487B]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Kostlánová, Nikola Wimmerová, Michaela Kyseľ, Peter Rotková, Gabriela
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
http://localhost/t...ganizacniJednotka	14740

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