About: Investigation of Matrix Deposition Techniques for High Throughput MALDI MS

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is an emerging technique with an immense biochemical and pharmaceutical potential, which possesses ability to show spatial distribution of specific compounds in tissues. The crucial step of MALDI-MSI experiment is matrix deposition. The MALDI matrix together with rhodamine B labeled angiotensin I was deposited on sample MALDI plate by three different approaches. By electrospray, spin-coating and piezoelectric nano-spotter. The homogeneity of deposited samples was studied under microscope by observing simple optical image and by observing fluorescence image after irradiating the sample with 532 nm laser. Optical image was compared with the MSI generated map of peptide distribution along the samples. To measure spatial distribution of selected peptides, a laboratory-built high-throughput axial time-of-flight (TOF) mass spectrometer with 2 kHz 355 nm Nd:YAG laser was used. Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is an emerging technique with an immense biochemical and pharmaceutical potential, which possesses ability to show spatial distribution of specific compounds in tissues. The crucial step of MALDI-MSI experiment is matrix deposition. The MALDI matrix together with rhodamine B labeled angiotensin I was deposited on sample MALDI plate by three different approaches. By electrospray, spin-coating and piezoelectric nano-spotter. The homogeneity of deposited samples was studied under microscope by observing simple optical image and by observing fluorescence image after irradiating the sample with 532 nm laser. Optical image was compared with the MSI generated map of peptide distribution along the samples. To measure spatial distribution of selected peptides, a laboratory-built high-throughput axial time-of-flight (TOF) mass spectrometer with 2 kHz 355 nm Nd:YAG laser was used. (en)
Title	Investigation of Matrix Deposition Techniques for High Throughput MALDI MS Investigation of Matrix Deposition Techniques for High Throughput MALDI MS (en)
skos:prefLabel	Investigation of Matrix Deposition Techniques for High Throughput MALDI MS Investigation of Matrix Deposition Techniques for High Throughput MALDI MS (en)
skos:notation	RIV/00216224:14740/11:00050182!RIV12-GA0-14740___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GCP206/10/J012), S, Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Tomalová, Iva Preisler, Jan Ertl, Lukáš Bednařík, Antonín
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	205708
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00050182
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	mass spectrometry imaging; matrix deposition; spincoating; electrospray; piezo actuated spotting (en)
http://linked.open.../riv/klicoveSlovo	electrospray mass spectrometry imaging matrix deposition piezo actuated spotting spincoating
http://linked.open...ontrolniKodProRIV	[2F9CBBD0AC33]
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Analysis of Biomolecules with Nanomaterials Assisted Laser Desorption/Ionization Mass Spectrometry
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Preisler, Jan Tomalová, Iva Ertl, Lukáš Bednařík, Antonín Moskovets, Eugene Rážová, Iva
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14740

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