About: Protein engineering of lectins - in silico and in vitro approaches

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	rotein-carbohydrate interactions are usually characterized by a low affinity for monovalent ligands that is balanced by multivalency resulting in high avidity for ligands with several potential epitops. However, recent characterization of lectins involved in pathogenesis has demonstrated their much higher affinity even towards monosaccharide than that observed for plant or animal lectins [1]. This feature allows to use such lectins as a more/less specific tool, for example, for evaluation of protein glycosylation or isolation of glyco-conjugates. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. In our work several methodical directions have been applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA. rotein-carbohydrate interactions are usually characterized by a low affinity for monovalent ligands that is balanced by multivalency resulting in high avidity for ligands with several potential epitops. However, recent characterization of lectins involved in pathogenesis has demonstrated their much higher affinity even towards monosaccharide than that observed for plant or animal lectins [1]. This feature allows to use such lectins as a more/less specific tool, for example, for evaluation of protein glycosylation or isolation of glyco-conjugates. Random mutagenesis is often used in protein engineering for producing proteins with altered or improved properties. In our work several methodical directions have been applied. Error-prone PCR is based on error-prone DNA polymerase which introduces nucleotide changes during extension of amplified DNA. (en)
Title	Protein engineering of lectins - in silico and in vitro approaches Protein engineering of lectins - in silico and in vitro approaches (en)
skos:prefLabel	Protein engineering of lectins - in silico and in vitro approaches Protein engineering of lectins - in silico and in vitro approaches (en)
skos:notation	RIV/00216224:14740/11:00050180!RIV12-GA0-14740___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP207/10/0321), P(GPP207/11/P185), Z(MSM0021622413)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Pokorná, Martina Adam, Jan Koča, Jaroslav Wimmerová, Michaela Mrázková, Jana
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	224813
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00050180
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lectin; error prone PCR; random mutagenesis (en)
http://linked.open.../riv/klicoveSlovo	lectin error prone PCR random mutagenesis
http://linked.open...ontrolniKodProRIV	[B68CC778CCD3]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Preparation of lectins with high specificity and affinity The ways to N-acetyl-mannosamine structures via mannoside metabolizing enzymes
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Adam, Jan Koča, Jaroslav Mrázková, Jana Pokorná, Martina Wimmerová, Michaela
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím
http://localhost/t...ganizacniJednotka	14740

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