About: Production of Ca-dependent high-affinity lectins with defined specificity by mutagenesis of PA-IIL lectin

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About: Production of Ca-dependent high-affinity lectins with defined specificity by mutagenesis of PA-IIL lectin Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The bacterium Pseudomonas aeruginosa is a human opportunistic pathogen that can infect almost every human tissue in conditions of a lowered immunity barrier. The lectin PA IIL plays an important role in the bacteria’s virulence. It displays unusually high affinity for L fucose in the micromolar range. [1] This characteristic is correlated with the remarkable presence of two calcium ions in the binding site of the protein. In vitro mutagenesis was performed focusing on three single point mutants of PA-IIL in positions 22-23-24. The mutated amino acids belong to the “specificity-binding loop”. The structure of all mutants was determined by X-ray crystallography and their thermodynamic characterization was performed using isothermal titration calorimetry. The mutants were designed also by in silico methods, and the resulting structures were used for docking experiments. The bacterium Pseudomonas aeruginosa is a human opportunistic pathogen that can infect almost every human tissue in conditions of a lowered immunity barrier. The lectin PA IIL plays an important role in the bacteria’s virulence. It displays unusually high affinity for L fucose in the micromolar range. [1] This characteristic is correlated with the remarkable presence of two calcium ions in the binding site of the protein. In vitro mutagenesis was performed focusing on three single point mutants of PA-IIL in positions 22-23-24. The mutated amino acids belong to the “specificity-binding loop”. The structure of all mutants was determined by X-ray crystallography and their thermodynamic characterization was performed using isothermal titration calorimetry. The mutants were designed also by in silico methods, and the resulting structures were used for docking experiments. (en)
Title	Production of Ca-dependent high-affinity lectins with defined specificity by mutagenesis of PA-IIL lectin Production of Ca-dependent high-affinity lectins with defined specificity by mutagenesis of PA-IIL lectin (en)
skos:prefLabel	Production of Ca-dependent high-affinity lectins with defined specificity by mutagenesis of PA-IIL lectin Production of Ca-dependent high-affinity lectins with defined specificity by mutagenesis of PA-IIL lectin (en)
skos:notation	RIV/00216224:14740/11:00050178!RIV12-GA0-14740___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GPP207/11/P185)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Pokorná, Martina Adam, Jan Koča, Jaroslav Wimmerová, Michaela
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	224262
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00050178
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lectin; Pseudomonas aeruginosa; PA-IIL; mutagenesis (en)
http://linked.open.../riv/klicoveSlovo	mutagenesis Pseudomonas aeruginosa lectin PA-IIL
http://linked.open...ontrolniKodProRIV	[34C632FDECD9]
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Preparation of lectins with high specificity and affinity
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Adam, Jan Imberty, Anne Koča, Jaroslav Pokorná, Martina Wimmerová, Michaela Mitchell, Edward P. Sabin, Charles
http://localhost/t...ganizacniJednotka	14740

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