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rdf:type
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Description
| - Directed evolution is widely used in protein engineering for producing proteins with altered or improved properties. This powerful tool includes several main approaches. The first is a random mutagenesis, which is used for creating random mutations anywhere in the gene of interest. The second is a targeted random mutagenesis, which is utilized for preparing mutants with random base substitutions in selected hotspots. In addition, a site directed mutagenesis is based on rational design and it serves for creating specific amino acid substitutions in selected hotspots. In our work two technical approaches - the targeted-random mutagenesis via mixture of oligonucleotide primers and the site directed mutagenesis via two primers carrying specific substitution – are employed. Lectins are proteins produced by different organisms. They are able to bind various sugars specifically.
- Directed evolution is widely used in protein engineering for producing proteins with altered or improved properties. This powerful tool includes several main approaches. The first is a random mutagenesis, which is used for creating random mutations anywhere in the gene of interest. The second is a targeted random mutagenesis, which is utilized for preparing mutants with random base substitutions in selected hotspots. In addition, a site directed mutagenesis is based on rational design and it serves for creating specific amino acid substitutions in selected hotspots. In our work two technical approaches - the targeted-random mutagenesis via mixture of oligonucleotide primers and the site directed mutagenesis via two primers carrying specific substitution – are employed. Lectins are proteins produced by different organisms. They are able to bind various sugars specifically. (en)
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Title
| - Directed evolution of lectins from pathogenic bacteria
- Directed evolution of lectins from pathogenic bacteria (en)
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skos:prefLabel
| - Directed evolution of lectins from pathogenic bacteria
- Directed evolution of lectins from pathogenic bacteria (en)
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skos:notation
| - RIV/00216224:14740/11:00050138!RIV12-GA0-14740___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA303/09/1168), P(GD301/09/H004), P(LC06030), S, Z(MSM0021622413)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14740/11:00050138
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - directed evolution; random mutagenesis in vitro; targeted-random mutagenesis; site-directed mutagenersis; lectins; PA-IIL; Pseudomonas aeruginosa; RS-IIL; Ralstonia solanacearum; BC2L-A; Burkholderia cenocepacia (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Mrázková, Jana
- Pokorná, Martina
- Wimmerová, Michaela
- Safy, Marek
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http://linked.open...n/vavai/riv/zamer
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http://localhost/t...ganizacniJednotka
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