About: CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study, CE-LIF with 405 nm laser diode was used to study AHP5 fused with GFP. AHP5-GFP fusion proteins overexpressed in E. coli were used for initial optimization of CE-LIF technique because of lower concentrations of the fusion protein produced by plant. The stability of the AHP5-GFP sample was examined; the complex was found to be unstable with GFP being released. The GFP release was not affected by either a long-term storage at -80 and -20 C or the addition of one of the inhibitors (EDTA, PMSF). These results were confirmed by the western blotting. The phosphorylation of the sample AHP5-GFP in vitro is under investigation. Gel filtration was used to obtain mass fractions for better understanding electrophoregrams. The mass fractions were investigated by CE-LIF and Western blotting-LIF and Western blotting. In this study, CE-LIF with 405 nm laser diode was used to study AHP5 fused with GFP. AHP5-GFP fusion proteins overexpressed in E. coli were used for initial optimization of CE-LIF technique because of lower concentrations of the fusion protein produced by plant. The stability of the AHP5-GFP sample was examined; the complex was found to be unstable with GFP being released. The GFP release was not affected by either a long-term storage at -80 and -20 C or the addition of one of the inhibitors (EDTA, PMSF). These results were confirmed by the western blotting. The phosphorylation of the sample AHP5-GFP in vitro is under investigation. Gel filtration was used to obtain mass fractions for better understanding electrophoregrams. The mass fractions were investigated by CE-LIF and Western blotting-LIF and Western blotting. (en)
Title	CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation (en)
skos:prefLabel	CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation CE-LIF of Green Fluorescent Fusion Proteins for Investigation of Phosphorylation (en)
skos:notation	RIV/00216224:14740/11:00050124!RIV12-GA0-14740___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GA203/09/1025), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Michalcová, Lenka Ryvolová, Markéta Hejátko, Jan Preisler, Jan Válková, Martina Dopitová, Radka
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	189364
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00050124
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CE-LIF; phosphorylation; AHP proteins (en)
http://linked.open.../riv/klicoveSlovo	AHP proteins CE-LIF phosphorylation
http://linked.open...ontrolniKodProRIV	[AC4A187593FC]
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Multidetection platform for microcolumn separation of proteins and peptides
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Dopitová, Radka Hejátko, Jan Michalcová, Lenka Preisler, Jan Ryvolová, Markéta Válková, Martina
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14740

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software