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rdf:type
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Description
| - The structure of N-terminal domain was determined by NMR. It consists mainly of three alpha-helixes and one short beta-sheet, yet the N-terminal part remains unstructured. The cause of this flexibility is probably the His6-Tag attached at the N-terminus.The N-terminal domain of delta subunit was conquested to high-throughput screening (sitting drop), where several crystallization conditions were found.Higher quality crystals were obtained after 7-9 days with an average size 150 x 100 micrometers.Diffraction data were collected at ESRF Grenoble, ID-23. The resolution of the structure was 3.5 Angstrom. The data were processed by MOSFLM and the determination of the structure was done by molecular replacement with NMR structure used as a model.
- The structure of N-terminal domain was determined by NMR. It consists mainly of three alpha-helixes and one short beta-sheet, yet the N-terminal part remains unstructured. The cause of this flexibility is probably the His6-Tag attached at the N-terminus.The N-terminal domain of delta subunit was conquested to high-throughput screening (sitting drop), where several crystallization conditions were found.Higher quality crystals were obtained after 7-9 days with an average size 150 x 100 micrometers.Diffraction data were collected at ESRF Grenoble, ID-23. The resolution of the structure was 3.5 Angstrom. The data were processed by MOSFLM and the determination of the structure was done by molecular replacement with NMR structure used as a model. (en)
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Title
| - Crystallization and structure determination of delta-subunit of RNApolymerase
- Crystallization and structure determination of delta-subunit of RNApolymerase (en)
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skos:prefLabel
| - Crystallization and structure determination of delta-subunit of RNApolymerase
- Crystallization and structure determination of delta-subunit of RNApolymerase (en)
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skos:notation
| - RIV/00216224:14740/11:00049774!RIV12-GA0-14740___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA204/09/0583), P(GD301/09/H004), Z(MSM0021622413)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14740/11:00049774
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - delta-subunit RNA polymerase crystallization structure (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Demo, Gabriel
- Papoušková, Veronika
- Wimmerová, Michaela
- Šanderová, Hana
- Žídek, Lukáš
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http://linked.open...n/vavai/riv/zamer
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http://localhost/t...ganizacniJednotka
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