About: Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein

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About: Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Non-coding RNA polymerase II transcripts are processed by the poly(A) independent termination pathway that requires the Nrd1 complex. The Nrd1 complex includes two RNA-binding proteins, the nuclear polyadenylated RNA-binding (Nab)3 and the nuclear pre-mRNA down-regulation (Nrd)1 that bind their specific termination elements. Here we report the solution structure of the RNA-recognition motif (RRM) of Nab3 in complex with a UCUU oligonucleotide, representing the Nab3 termination element. The structure shows that the first three nucleotides of UCUU are accommodated on the beta-sheet surface of Nab3 RRM, but reveals a sequence specific recognition only for the central cytidine and uridine. The specific contacts we identified are important for binding affinity in vitro as well as for yeast viability. Further, we show that both RNA-binding motifs of Nab3 and Nrd1 alone bind their termination elements with a weak affinity. Non-coding RNA polymerase II transcripts are processed by the poly(A) independent termination pathway that requires the Nrd1 complex. The Nrd1 complex includes two RNA-binding proteins, the nuclear polyadenylated RNA-binding (Nab)3 and the nuclear pre-mRNA down-regulation (Nrd)1 that bind their specific termination elements. Here we report the solution structure of the RNA-recognition motif (RRM) of Nab3 in complex with a UCUU oligonucleotide, representing the Nab3 termination element. The structure shows that the first three nucleotides of UCUU are accommodated on the beta-sheet surface of Nab3 RRM, but reveals a sequence specific recognition only for the central cytidine and uridine. The specific contacts we identified are important for binding affinity in vitro as well as for yeast viability. Further, we show that both RNA-binding motifs of Nab3 and Nrd1 alone bind their termination elements with a weak affinity. (en)
Title	Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein (en)
skos:prefLabel	Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein (en)
skos:notation	RIV/00216224:14740/11:00049398!RIV12-GA0-14740___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/08/1212), P(GAP305/10/1490), P(GD204/08/H054), P(IAA401630903), P(LA08008), Z(MSM0021622413), Z(MSM0021622415)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kubíček, Karel Bačíková, Veronika Vaňáčová, Štěpánka Štefl, Richard Pasulka, Josef Hroššová, Dominika Hofr, Ctirad Zimmermann, Michal Hóbor, Fruzsina Pergoli, Roberto
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	225956
http://linked.open...ai/riv/idVysledku	RIV/00216224:14740/11:00049398
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	NMR; Protein structure; RNA processing; RNA-protein interaction; Transcription termination (en)
http://linked.open.../riv/klicoveSlovo	NMR Protein structure RNA processing RNA-protein interaction Transcription termination
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C5B7441028E8]
http://linked.open...i/riv/nazevZdroje	The Journal of Biological Chemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	10 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular mechanisms of the cell proliferation and differentiation Structural studies of protein-RNA complexes involved in RNA quality control Structural basis for poly(A) independent transcription termination pathway Structural basis for transcription termination of nonpolyadenylated transcripts Structural studies of protein-RNA complexes involved in RNA quality control
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	286
http://linked.open...iv/tvurceVysledku	Kubíček, Karel Bačíková, Veronika Hofr, Ctirad Hroššová, Dominika Hóbor, Fruzsina Pasulka, Josef Pergoli, Roberto Vaňáčová, Štěpánka Zimmermann, Michal Štefl, Richard
http://linked.open...ain/vavai/riv/wos	000286653200048
http://linked.open...n/vavai/riv/zamer	Proteiny v metabolismu a při interakci organismů s prostředím Molecular basis of cell and tissue regulations
issn	0021-9258
number of pages	13 (xsd:int)
http://bibframe.org/vocab/doi	10.1074/jbc.M110.158774
http://localhost/t...ganizacniJednotka	14740

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