About: Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions     Goto   Sponge   NotDistinct   Permalink

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  • Electron tomographic reconstructions suffer from a number of artefacts arising from effects accompanying the processes of acquisition of a set of tilted projections of the specimen in a transmission electron microscope and from its subsequent computational handling. The most pronounced artefacts usually come from imprecise projection alignment, distortion of specimens during tomogram acquisition and from the presence of a region of missing data in the Fourier space, the %22missing wedge%22. The ray artefacts caused by the presence of the missing wedge can be attenuated by the angular image filter, which attenuates the transition between the data and the missing wedge regions. In this work, we present an analysis of the influence of angular filtering on the resolution of averaged repetitive structural motives extracted from three-dimensional reconstructions of tomograms acquired in the single-axis tilting geometry.
  • Electron tomographic reconstructions suffer from a number of artefacts arising from effects accompanying the processes of acquisition of a set of tilted projections of the specimen in a transmission electron microscope and from its subsequent computational handling. The most pronounced artefacts usually come from imprecise projection alignment, distortion of specimens during tomogram acquisition and from the presence of a region of missing data in the Fourier space, the %22missing wedge%22. The ray artefacts caused by the presence of the missing wedge can be attenuated by the angular image filter, which attenuates the transition between the data and the missing wedge regions. In this work, we present an analysis of the influence of angular filtering on the resolution of averaged repetitive structural motives extracted from three-dimensional reconstructions of tomograms acquired in the single-axis tilting geometry. (en)
Title
  • Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions
  • Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions (en)
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  • Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions
  • Sub-Volume Averaging of Repetitive Structural Features in Angularly Filtered Electron Tomographic Reconstructions (en)
skos:notation
  • RIV/00216224:14330/14:00080247!RIV15-MSM-14330___
http://linked.open...avai/riv/aktivita
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  • I, P(ED1.1.00/02.0109), P(GBP302/12/G157), P(GP13-32339P)
http://linked.open...iv/cisloPeriodika
  • Suppl. 1
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  • 48485
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  • RIV/00216224:14330/14:00080247
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  • electron tomography; missing wedge; limited specimen tilting; sub-volume averaging (en)
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  • US - Spojené státy americké
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  • [E5AE81E4A28C]
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  • Folia biologica
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http://linked.open...v/svazekPeriodika
  • 60
http://linked.open...iv/tvurceVysledku
  • Matula, Pavel
  • Raška, Ivan
  • Kereiche, Sami
  • Kovacik, Lubomír
http://linked.open...ain/vavai/riv/wos
  • 000343275800011
issn
  • 0015-5500
number of pages
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  • 14330
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