About: HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs

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About: HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin. Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin. (en)
Title	HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs (en)
skos:prefLabel	HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs (en)
skos:notation	RIV/00216224:14330/14:00074061!RIV15-GA0-14330___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GBP302/12/G157)
http://linked.open...iv/cisloPeriodika	39
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Matula, Pavel Sorokin, Dmitry
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	19836
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/14:00074061
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA-damage response; DNA repair; Irradiation; Live-cell studies; Nucleolus; UBF1 (en)
http://linked.open.../riv/klicoveSlovo	Irradiation DNA-damage response Nucleolus DNA repair Live-cell studies UBF1
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[74D9ED7CDA8C]
http://linked.open...i/riv/nazevZdroje	Epigenetics & Chromatin
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	12 (xsd:int)
http://linked.open...vavai/riv/projekt	Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Bártová, Eva Fulneček, Jaroslav Kozubek, Stanislav Kovařík, Aleš Matula, Pavel Raška, Ivan Legartová, Soňa Stixová, Lenka Suchánková, Jana Sorokin, Dmitry Hrušková, Tereza Sehnalová, Petra
http://linked.open...ain/vavai/riv/wos	000347931500001
issn	1756-8935
number of pages	17 (xsd:int)
http://bibframe.org/vocab/doi	10.1186/1756-8935-7-39
http://localhost/t...ganizacniJednotka	14330

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