About: Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy

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About: Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	To overcome anisotropic optical resolution or point localization precision in 3D fluorescence microscopy, micro axial tomography was suggested which allows object tilting on the microscopic stage. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. Test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range using standard microscopic equipment. To overcome anisotropic optical resolution or point localization precision in 3D fluorescence microscopy, micro axial tomography was suggested which allows object tilting on the microscopic stage. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. Test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range using standard microscopic equipment. (en)
Title	Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy (en)
skos:prefLabel	Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy (en)
skos:notation	RIV/00216224:14330/11:00053149!RIV12-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), Z(MSM0021622419)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Kozubek, Michal Matula, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	212282
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/11:00053149
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence; glass fibres; image reconstruction; image resolution; optical microscopes; optical microscopy; optical tomography; stepping motors (en)
http://linked.open.../riv/klicoveSlovo	fluorescence image reconstruction optical microscopy glass fibres image resolution optical microscopes optical tomography stepping motors
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[F3FE0EA1E0C7]
http://linked.open...i/riv/nazevZdroje	Rev. Sci. Instrum.
http://linked.open...in/vavai/riv/obor	JD
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	82
http://linked.open...iv/tvurceVysledku	Kozubek, Michal Matula, Petr Cremer, Christoph Eipel, Heinz Evsikov, Alexei V Hausmann, Michael Staier, Florian
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
issn	0034-6748
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

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