About: Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology

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About: Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Using time-lapse microscopy, protein localization and dynamics can be studied directly in living cells. This may bring significant information necessary to complete understanding of protein function. Aberrant tyrosinkinase Bcr/Abl is the main cause of chronic myelogenous leukemia. Abl1 tyrosinkinase represents its native form. Among others these natural and aberrant kinase differ in cell localization, which will be the subject for subsequent examination in the K562 leukemic cell line or in CD34+ cells from bone marrow. We created a unique set of Bcr/Abl and Abl1 expression vectors tagged with EYFP or RFP sequences for examination of protein pools movements in the living cells. The Gateway cloning system was chosen for its flexibility and robustness. We applied two types of fluorescent protein to be able watch both forms of kinase simultaneously. Using time-lapse microscopy, protein localization and dynamics can be studied directly in living cells. This may bring significant information necessary to complete understanding of protein function. Aberrant tyrosinkinase Bcr/Abl is the main cause of chronic myelogenous leukemia. Abl1 tyrosinkinase represents its native form. Among others these natural and aberrant kinase differ in cell localization, which will be the subject for subsequent examination in the K562 leukemic cell line or in CD34+ cells from bone marrow. We created a unique set of Bcr/Abl and Abl1 expression vectors tagged with EYFP or RFP sequences for examination of protein pools movements in the living cells. The Gateway cloning system was chosen for its flexibility and robustness. We applied two types of fluorescent protein to be able watch both forms of kinase simultaneously. (en)
Title	Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology (en)
skos:prefLabel	Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology Time lapse living cells microscopy for studying CML: Preparation of Abl1- and Bcr/Abl - fluorescent protein vectors using Gateway technology (en)
skos:notation	RIV/00216224:14330/10:00045378!RIV11-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), Z(MSM0021622430)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Vařecha, Miroslav Potěšilová, Michaela Krontorád Koutná, Irena Tesařová, Lenka
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	292906
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/10:00045378
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Gateway cloning ABL1 BCR/ABL expression vectors (en)
http://linked.open.../riv/klicoveSlovo	Gateway cloning ABL1 BCR/ABL expression vectors
http://linked.open...ontrolniKodProRIV	[96103C9B1EDA]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	Advances in Molecular and Cancer Biology, 2010
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Potěšilová, Michaela Vařecha, Miroslav Krontorád Koutná, Irena Tesařová, Lenka
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2010-11-11 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
number of pages	5 (xsd:int)
http://purl.org/ne...btex#hasPublisher	muni PRESS
https://schema.org/isbn	978-80-210-5312-0
http://localhost/t...ganizacniJednotka	14330

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