About: Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire

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About: Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Monitoring the T-cell population diversity has demonstrated to be beneficial when evaluating an individuals immune status. BV families are most often analysed to examine this diversity. The aim of our study was to establish a method for specific determination of relative BV family expression and to evaluate its applicability in terms of multiple myeloma (MM). Peripheral blood mononuclear cells from healthy donors and MM patients were analysed by RT-PCR. PCR product amounts were determined with an Agilent 2100 Bioanalyzer utilizing capillary electrophoresis and converted to the relative BV family expression. This report provides reverse transcription PCR with primers allowing for the analysis of the entire BV family repertoire. Further it describes the utilization of capillary electrophoresis to evaluate the relative BV family expression after their amplification. The methodology is fast and simple to operate and is therefore suitable as a first screening of the T-cell population composition. Monitoring the T-cell population diversity has demonstrated to be beneficial when evaluating an individuals immune status. BV families are most often analysed to examine this diversity. The aim of our study was to establish a method for specific determination of relative BV family expression and to evaluate its applicability in terms of multiple myeloma (MM). Peripheral blood mononuclear cells from healthy donors and MM patients were analysed by RT-PCR. PCR product amounts were determined with an Agilent 2100 Bioanalyzer utilizing capillary electrophoresis and converted to the relative BV family expression. This report provides reverse transcription PCR with primers allowing for the analysis of the entire BV family repertoire. Further it describes the utilization of capillary electrophoresis to evaluate the relative BV family expression after their amplification. The methodology is fast and simple to operate and is therefore suitable as a first screening of the T-cell population composition. (en)
Title	Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire (en)
skos:prefLabel	Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire Application of reverse transcription PCR and capillary electrophoresis in the analysis of T-cell receptor beta variable repertoire (en)
skos:notation	RIV/00216224:14330/10:00045361!RIV11-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), Z(MSM0021622430)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Potěšilová, Michaela Stejskal, Stanislav Krontorád Koutná, Irena Tesařová, Lenka Peterková, Martina
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	247486
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/10:00045361
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Reverse transcription polymerase chain reaction; Capillary electrophoresis; T-cell receptor; Beta variable families (en)
http://linked.open.../riv/klicoveSlovo	Capillary electrophoresis Beta variable families Reverse transcription polymerase chain reaction T-cell receptor
http://linked.open...ontrolniKodProRIV	[6C9113300A08]
http://linked.open...v/mistoKonaniAkce	Brno, Česká Republika
http://linked.open...i/riv/mistoVydani	Brno, Česká republika
http://linked.open...i/riv/nazevZdroje	Advances in Molecular and Cancer Biology, 2010
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Potěšilová, Michaela Stejskal, Stanislav Krontorád Koutná, Irena Tesařová, Lenka Peterková, Martina
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2010-11-11 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
number of pages	6 (xsd:int)
http://purl.org/ne...btex#hasPublisher	muni PRESS
https://schema.org/isbn	978-80-210-5312-0
http://localhost/t...ganizacniJednotka	14330

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