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  • This contribution shows how to modify classical autofocusing in optical microscopy to perform automated analysis of information content in image data generated by the instrument, even if dealing with thick specimen or working in confocal mode. The new approach is useful for finding 3D regions of interest prior to image acquisition, which enables the user to record precisely those places in space that contain useful image data. This results in shorter acquisition time and less memory consumption as compared to the acquisition of classical large rectangular volumes containing more background regions than object regions (especially for a sparse specimen and camera-based systems). The standard process of image focusing assumes unimodal behaviour of the focus function. In real world, however, this assumption is often not fulfilled and the objects normally do not lie in one plane.
  • This contribution shows how to modify classical autofocusing in optical microscopy to perform automated analysis of information content in image data generated by the instrument, even if dealing with thick specimen or working in confocal mode. The new approach is useful for finding 3D regions of interest prior to image acquisition, which enables the user to record precisely those places in space that contain useful image data. This results in shorter acquisition time and less memory consumption as compared to the acquisition of classical large rectangular volumes containing more background regions than object regions (especially for a sparse specimen and camera-based systems). The standard process of image focusing assumes unimodal behaviour of the focus function. In real world, however, this assumption is often not fulfilled and the objects normally do not lie in one plane. (en)
Title
  • Information content analysis in automated microscopy imaging using modified autofocusing approach
  • Information content analysis in automated microscopy imaging using modified autofocusing approach (en)
skos:prefLabel
  • Information content analysis in automated microscopy imaging using modified autofocusing approach
  • Information content analysis in automated microscopy imaging using modified autofocusing approach (en)
skos:notation
  • RIV/00216224:14330/10:00044036!RIV11-MSM-14330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(2B06052), P(LC535), S, Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 263952
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14330/10:00044036
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • automated microscopy; autofocusing; 3D information content analysis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [A7B27D7C6E1B]
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Kozubek, Michal
  • Brázdilová, Silvie Luisa
http://linked.open...n/vavai/riv/zamer
http://localhost/t...ganizacniJednotka
  • 14330
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