About: Automated spinning disk confocal microscopy in 3D live cell imaging

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fluorescence microscopy has become the leading technology to study structure and dynamics of cellular components and processes. Fluorescent proteins allow us to study protein dynamics, localization, and interactions in living cells. In our laboratory, we have been developing special systems for automated cell image acquisition and analysis using fluorescence microscopy working up to five dimensions (x, y, z, t, lambda), whose hardware and software was optimized for studies on living cells. The presentation will focus on the latest developments in our technology. Besides microscopy hardware and software, also examples of possible applications will be presented. For the first time, we discovered interaction of apoptotic proteins AIF and endonuclease G, expressed using one DNA plasmid, in living human cells during apoptotic cell death. Fluorescence microscopy has become the leading technology to study structure and dynamics of cellular components and processes. Fluorescent proteins allow us to study protein dynamics, localization, and interactions in living cells. In our laboratory, we have been developing special systems for automated cell image acquisition and analysis using fluorescence microscopy working up to five dimensions (x, y, z, t, lambda), whose hardware and software was optimized for studies on living cells. The presentation will focus on the latest developments in our technology. Besides microscopy hardware and software, also examples of possible applications will be presented. For the first time, we discovered interaction of apoptotic proteins AIF and endonuclease G, expressed using one DNA plasmid, in living human cells during apoptotic cell death. (en)
Title	Automated spinning disk confocal microscopy in 3D live cell imaging Automated spinning disk confocal microscopy in 3D live cell imaging (en)
skos:prefLabel	Automated spinning disk confocal microscopy in 3D live cell imaging Automated spinning disk confocal microscopy in 3D live cell imaging (en)
skos:notation	RIV/00216224:14330/10:00043375!RIV11-MSM-14330___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(2B06052), P(LC535), S, Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Vařecha, Miroslav Kozubek, Michal Matula, Pavel
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	304459
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/10:00043375
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	confocal microscopy; living cells; fluorescence (en)
http://linked.open.../riv/klicoveSlovo	living cells fluorescence confocal microscopy
http://linked.open...ontrolniKodProRIV	[833C3E253BC5]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Matula, Pavel Kozubek, Michal Vařecha, Miroslav
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
http://localhost/t...ganizacniJednotka	14330

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