About: Combination of mRNA and protein microarray analysis in complex cell profiling

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This study combines mRNA and protein analysis using cDNA and antibody microarray techniques, respectively. These create a novel, integrated perspective into cellular molecular profiles. The aims of this study were to establish a reliable way of integrating these two approaches in order to obtain complex molecular profiles of the cell and to find suitable methods to normalize the data obtained using these approaches. Antibody microarray and cDNA microarray techniques were used to study expression alterations in HL-60 cells that were differentiated into granulocytes using all-trans retinoic acid (ATRA). We selected this model to evaluate this combined profiling technique because the expression levels of most of the mRNA and protein species in these cells are not altered; therefore it is easier to track and define those species that are changed. The proteins whose levels were altered included cmyc, c-jun, Pyk2, FAK, PKC, TRF1, NF-kappaB and certain caspase types. This study combines mRNA and protein analysis using cDNA and antibody microarray techniques, respectively. These create a novel, integrated perspective into cellular molecular profiles. The aims of this study were to establish a reliable way of integrating these two approaches in order to obtain complex molecular profiles of the cell and to find suitable methods to normalize the data obtained using these approaches. Antibody microarray and cDNA microarray techniques were used to study expression alterations in HL-60 cells that were differentiated into granulocytes using all-trans retinoic acid (ATRA). We selected this model to evaluate this combined profiling technique because the expression levels of most of the mRNA and protein species in these cells are not altered; therefore it is easier to track and define those species that are changed. The proteins whose levels were altered included cmyc, c-jun, Pyk2, FAK, PKC, TRF1, NF-kappaB and certain caspase types. (en)
Title	Combination of mRNA and protein microarray analysis in complex cell profiling Combination of mRNA and protein microarray analysis in complex cell profiling (en)
skos:prefLabel	Combination of mRNA and protein microarray analysis in complex cell profiling Combination of mRNA and protein microarray analysis in complex cell profiling (en)
skos:notation	RIV/00216224:14330/09:00034277!RIV10-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	56
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Kozubek, Michal Ručka, Zdeněk Stejskal, Stanislav Šimara, Pavel Krontorád Koutná, Irena Peterková, Martina Krontorád, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	307527
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/09:00034277
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	microarray; cell profiling; protein expression; mRNA expression; HL-60 (en)
http://linked.open.../riv/klicoveSlovo	HL-60 mRNA expression microarray protein expression cell profiling
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[9E51CE8BA297]
http://linked.open...i/riv/nazevZdroje	Neoplasma
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	2/2009
http://linked.open...iv/tvurceVysledku	Kozubek, Michal Stejskal, Stanislav Šimara, Pavel Krontorád Koutná, Irena Peterková, Martina Ručka, Zdeněk Krontorád, Petr
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	0028-2685
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

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