About: Single-cell-based image analysis of high-throughput cell array screens for quantification of viral infection

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About: Single-cell-based image analysis of high-throughput cell array screens for quantification of viral infection Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The identification of eukaryotic genes involved in virus entry and replication is important for understanding viral infection. Our goal is to develop a siRNA-based screening system using cell arrays and high-throughput (HT) fluorescence microscopy. A central issue is efficient, robust, and automated single-cell-based analysis of massive image datasets. We have developed an image analysis approach that comprises (i) a novel, gradient-based thresholding scheme for cell nuclei segmentation which does not require subsequent postprocessing steps for separation of clustered nuclei, (ii) quantification of the virus signal in the neighborhood of cell nuclei, (iii) localization of regions with transfected cells by combining model-based circle fitting and grid fitting, (iv) cell classification as infected or noninfected, and (v) image quality control (e.g., identification of out-of-focus images). The identification of eukaryotic genes involved in virus entry and replication is important for understanding viral infection. Our goal is to develop a siRNA-based screening system using cell arrays and high-throughput (HT) fluorescence microscopy. A central issue is efficient, robust, and automated single-cell-based analysis of massive image datasets. We have developed an image analysis approach that comprises (i) a novel, gradient-based thresholding scheme for cell nuclei segmentation which does not require subsequent postprocessing steps for separation of clustered nuclei, (ii) quantification of the virus signal in the neighborhood of cell nuclei, (iii) localization of regions with transfected cells by combining model-based circle fitting and grid fitting, (iv) cell classification as infected or noninfected, and (v) image quality control (e.g., identification of out-of-focus images). (en)
Title	Single-cell-based image analysis of high-throughput cell array screens for quantification of viral infection Single-cell-based image analysis of high-throughput cell array screens for quantification of viral infection (en)
skos:prefLabel	Single-cell-based image analysis of high-throughput cell array screens for quantification of viral infection Single-cell-based image analysis of high-throughput cell array screens for quantification of viral infection (en)
skos:notation	RIV/00216224:14330/09:00034133!RIV10-MSM-14330___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(2B06052)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Matula, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	341423
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/09:00034133
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	image analysis; cell nucleus segmentation; quantification of viral infection; siRNA screening; cell-based arrays; immunofluorescence microscopy; image quality control (en)
http://linked.open.../riv/klicoveSlovo	cell nucleus segmentation cell-based arrays image quality control quantification of viral infection siRNA screening image analysis immunofluorescence microscopy
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[32E160A875B1]
http://linked.open...i/riv/nazevZdroje	Cytometry Part A
http://linked.open...in/vavai/riv/obor	JD
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	75A
http://linked.open...iv/tvurceVysledku	Matula, Petr Bartenschlager, Ralf Eils, Roland Kumar, Anil Rohr, Karl Erfle, Holger Wörz, Ilka
http://linked.open...ain/vavai/riv/wos	000264513800005
issn	1552-4922
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

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