About: cDNA Microarray: Gene Expression Profiling of Small Cell Populations

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to analyze more precisely defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The goal was to find the best method of isolating and amplifying RNA from a small number of cells. We used the HL60 cell line in the search for a suitable protocol that could be applied to clinical samples of CD34+ hematopoietic cells. These cells make up only a very small population in bone marrow (1- 4% of all cells). cDNA microarray technology is widely used in various biological and medical disciplines to determine gene expression profiles. Unfortunately, this technology requires a large quantity of input RNA. Since there is an increasing need to analyze more precisely defined cell sub-populations with low cell counts, working protocols using a minimal number of input cells are needed. Optimal RNA isolation and its accurate amplification are crucial to the success of these protocols. The goal was to find the best method of isolating and amplifying RNA from a small number of cells. We used the HL60 cell line in the search for a suitable protocol that could be applied to clinical samples of CD34+ hematopoietic cells. These cells make up only a very small population in bone marrow (1- 4% of all cells). (en)
Title	cDNA Microarray: Gene Expression Profiling of Small Cell Populations cDNA Microarray: Gene Expression Profiling of Small Cell Populations (en)
skos:prefLabel	cDNA Microarray: Gene Expression Profiling of Small Cell Populations cDNA Microarray: Gene Expression Profiling of Small Cell Populations (en)
skos:notation	RIV/00216224:14330/08:00027029!RIV10-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), P(LC535), Z(MSM0021622430)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Potěšilová, Michaela Hrabčáková, Viera Ručka, Zdeněk Krontorád Koutná, Irena Klabusay, Martin Tesařová, Lenka Peterková, Martina
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	358970
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/08:00027029
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cDNA microarray; RNA isolation; RNA amplifiacation; CD34+ cells (en)
http://linked.open.../riv/klicoveSlovo	CD34+ cells RNA isolation RNA amplifiacation cDNA microarray
http://linked.open...ontrolniKodProRIV	[40A2CA0B2606]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Potěšilová, Michaela Klabusay, Martin Krontorád Koutná, Irena Tesařová, Lenka Peterková, Martina Ručka, Zdeněk Hrabčáková, Viera
http://linked.open...n/vavai/riv/zamer	Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
http://localhost/t...ganizacniJednotka	14330

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