About: Image analysis of localization and translocation of apoptotic proteins in living cells

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About: Image analysis of localization and translocation of apoptotic proteins in living cells Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in living cells using both single-cell colocalization image analysis and nuclear translocation image analysis. Image data were acquired using automated fluorescent microscope Zeiss Axiovert 200M with spinning disc confocal unit Yokogawa CSU-10. We confirmed the mitochondrial localization of endonuclease G and AIF by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF. We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in living cells using both single-cell colocalization image analysis and nuclear translocation image analysis. Image data were acquired using automated fluorescent microscope Zeiss Axiovert 200M with spinning disc confocal unit Yokogawa CSU-10. We confirmed the mitochondrial localization of endonuclease G and AIF by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF. (en)
Title	Image analysis of localization and translocation of apoptotic proteins in living cells Image analysis of localization and translocation of apoptotic proteins in living cells (en)
skos:prefLabel	Image analysis of localization and translocation of apoptotic proteins in living cells Image analysis of localization and translocation of apoptotic proteins in living cells (en)
skos:notation	RIV/00216224:14330/08:00026068!RIV11-MSM-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC535), Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Amrichová, Jana Ulman, Vladimír Vařecha, Miroslav Kozubek, Michal Zimmermann, Michal
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	371406
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/08:00026068
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	AIF; endonuclease G; AMID; living cells; colocalization; image analysis (en)
http://linked.open.../riv/klicoveSlovo	endonuclease G living cells colocalization image analysis AIF AMID
http://linked.open...ontrolniKodProRIV	[E788C8CACD2A]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions
http://linked.open...UplatneniVysledku	2008
http://linked.open...iv/tvurceVysledku	Ulman, Vladimír Kozubek, Michal Vařecha, Miroslav Zimmermann, Michal Amrichová, Jana
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
http://localhost/t...ganizacniJednotka	14330

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