About: Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells

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About: Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	During apoptosis several mitochondrial proteins are released. They participate in caspase-independent nuclear DNA degradation, namely apoptosis-inducing factor (AIF, also PDCD8 or AIFM1) and endonuclease G. Another interesting protein, which is not located inside mitochondria, was expected to act similarly as AIF due to high sequence homology with AIF. This protein is AIF-homologous mitochondrion-associated inducer of death (AMID, also PRG3 or AIFM2). We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID experimentally using designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins and using bioinformatic predictions, that analyze the amino acid sequence of the proteins with various algorithms. We also designed and applied the novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus. During apoptosis several mitochondrial proteins are released. They participate in caspase-independent nuclear DNA degradation, namely apoptosis-inducing factor (AIF, also PDCD8 or AIFM1) and endonuclease G. Another interesting protein, which is not located inside mitochondria, was expected to act similarly as AIF due to high sequence homology with AIF. This protein is AIF-homologous mitochondrion-associated inducer of death (AMID, also PRG3 or AIFM2). We studied the cellular localization and colocalization of proteins AIF, endonuclease G and AMID experimentally using designed mammalian expression vectors, which carry genes encoding the proteins of interest fused to the fluorescent proteins and using bioinformatic predictions, that analyze the amino acid sequence of the proteins with various algorithms. We also designed and applied the novel method of single-cell image analysis of the translocation of the fluorescent proteins into the nucleus. (en)
Title	Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells (en)
skos:prefLabel	Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells (en)
skos:notation	RIV/00216224:14330/07:00020613!RIV11-GA0-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP204/05/P090), P(LC535), Z(MSM0021622419)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Amrichová, Jana Ulman, Vladimír Vařecha, Miroslav Kozubek, Michal Matula, Pavel Zimmermann, Michal
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	411849
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/07:00020613
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	apoptosis; cell death; autophagy; cancer (en)
http://linked.open.../riv/klicoveSlovo	cancer cell death apoptosis autophagy
http://linked.open...ontrolniKodProRIV	[411C706571A8]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Chromosome dynamics and organization during the cell cycle in normal and pathologic conditions Topographic and functional characterization of telomeres in various tumor cell lines determined in situ and in vivo
http://linked.open...UplatneniVysledku	2007
http://linked.open...iv/tvurceVysledku	Matula, Pavel Ulman, Vladimír Kozubek, Michal Vařecha, Miroslav Zimmermann, Michal Amrichová, Jana
http://linked.open...n/vavai/riv/zamer	Highly Parallel and Distributed Computing Systems
http://localhost/t...ganizacniJednotka	14330

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