About: Directional motion of foreign plasmid DNA to nuclear HP1 foci

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About: Directional motion of foreign plasmid DNA to nuclear HP1 foci Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Movement of labelled plasmid DNA relative to heterochromatin foci in nuclei, visualized with HP1-GFP, was studied using live-cell imaging and object tracking. In addition to Brownian motion of plasmid DNA we found a pronounced, non-random movement of plasmid DNA towards the nearest HP1 focus, while time-lapse microscopy showed that HP1 foci are relatively immobile and positionally stable. The movement of plasmid DNA was much faster than that of the HP1 foci. Contact of transgene DNA with an HP1 focus usually resulted in cessation of the directional motion. Moreover, the motion of plasmid DNA inside the heterochromatin compartment was more restricted (limited to 0.25 mu m) than when the plasmid DNA was outside heterochromatin (R = 0.7 mu m). Three days after transfection most of the foreign labelled DNA colocalized with centromeric heterochromatin. Movement of labelled plasmid DNA relative to heterochromatin foci in nuclei, visualized with HP1-GFP, was studied using live-cell imaging and object tracking. In addition to Brownian motion of plasmid DNA we found a pronounced, non-random movement of plasmid DNA towards the nearest HP1 focus, while time-lapse microscopy showed that HP1 foci are relatively immobile and positionally stable. The movement of plasmid DNA was much faster than that of the HP1 foci. Contact of transgene DNA with an HP1 focus usually resulted in cessation of the directional motion. Moreover, the motion of plasmid DNA inside the heterochromatin compartment was more restricted (limited to 0.25 mu m) than when the plasmid DNA was outside heterochromatin (R = 0.7 mu m). Three days after transfection most of the foreign labelled DNA colocalized with centromeric heterochromatin. (en)
Title	Directional motion of foreign plasmid DNA to nuclear HP1 foci Directional motion of foreign plasmid DNA to nuclear HP1 foci (en)
skos:prefLabel	Directional motion of foreign plasmid DNA to nuclear HP1 foci Directional motion of foreign plasmid DNA to nuclear HP1 foci (en)
skos:notation	RIV/00216224:14330/06:00040619!RIV11-AV0-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/02/0804), P(IAA1065203), Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Ondřej, Vladan Kozubek, Michal Kozubek, Stanislav Matula, Pavel Falk, Martin Matula, Petr Lukášová, Emilie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	471842
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/06:00040619
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	heterochromatin; HP1 protein; nuclear compartments; plasmid; transfection (en)
http://linked.open.../riv/klicoveSlovo	plasmid transfection HP1 protein heterochromatin nuclear compartments
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C51C6785D126]
http://linked.open...i/riv/nazevZdroje	Chromosome Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Chronic myeloid leukemia radiation risk estimation based on BCR-ABL distances in hemotopoietic cells The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Kozubek, Stanislav Lukášová, Emilie Matula, Pavel Ondřej, Vladan Falk, Martin Kozubek, Michal Matula, Petr
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems
issn	0967-3849
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

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