About: Obtaining DNA markers for diversity evaluation in Trifolium pratense cultivars

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Red clover (Trifolium pratense L.) is one of the main forage species of temperate regions. Cultivars of red clover are allogamous and heterogeneous which their genetic analysis makes difficult. The objective of this study was obtaining DNA markers (RAPD, SSR) for genetic diversity evaluation among red clover cultivars and verification of utility of SSR oligonucleotides from white clover (T. repens). RAPD (Random Amplified Polymorphic DNA) requires no knowledge of DNA sequences for primers designing. SSRs (Simple Sequence Repeats) are highly informative codominant molecular markers which are well known in white clover. Red clover cv. Kvarta and Start and white clover cv. Hájek and Jura were used for molecular analysis. Genomic DNA of red and white clover plants was extracted by bulking method from different plant numbers. CTAB-based method and GenEluteTMPlant Genomic DNA Kit (Sigma) were used for DNA isolation. Red clover (Trifolium pratense L.) is one of the main forage species of temperate regions. Cultivars of red clover are allogamous and heterogeneous which their genetic analysis makes difficult. The objective of this study was obtaining DNA markers (RAPD, SSR) for genetic diversity evaluation among red clover cultivars and verification of utility of SSR oligonucleotides from white clover (T. repens). RAPD (Random Amplified Polymorphic DNA) requires no knowledge of DNA sequences for primers designing. SSRs (Simple Sequence Repeats) are highly informative codominant molecular markers which are well known in white clover. Red clover cv. Kvarta and Start and white clover cv. Hájek and Jura were used for molecular analysis. Genomic DNA of red and white clover plants was extracted by bulking method from different plant numbers. CTAB-based method and GenEluteTMPlant Genomic DNA Kit (Sigma) were used for DNA isolation. (en)
Title	Obtaining DNA markers for diversity evaluation in Trifolium pratense cultivars Obtaining DNA markers for diversity evaluation in Trifolium pratense cultivars (en)
skos:prefLabel	Obtaining DNA markers for diversity evaluation in Trifolium pratense cultivars Obtaining DNA markers for diversity evaluation in Trifolium pratense cultivars (en)
skos:notation	RIV/00216224:14330/05:00012699!RIV10-MZE-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1G46034), P(GD204/05/H505), Z(MSM0021622415), Z(MSM2629608001)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Jungmannová, Barbara Řepková, Jana
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	533907
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/05:00012699
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA markers; microsatellites; Trifolium pratense (en)
http://linked.open.../riv/klicoveSlovo	DNA markers microsatellites Trifolium pratense
http://linked.open...ontrolniKodProRIV	[66F0B131A4B5]
http://linked.open...v/mistoKonaniAkce	České Budějovice
http://linked.open...i/riv/mistoVydani	České Budějovice
http://linked.open...i/riv/nazevZdroje	Book of Abstracts, 6th International Symposium in the Series Recent Advances in Plant Biotechnology, 12.-16.9.2005
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Introduction of new traits for quality and stability improving in variety production of selected leguminous species by hybridization methods Developmental and evolutionary genetics of plants
http://linked.open...UplatneniVysledku	2005
http://linked.open...iv/tvurceVysledku	Řepková, Jana Jungmannová, Barbara
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2005-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations Genetic-breeding and technological aspects of suistainable fodder crops production
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Ústav molekulární biologie rostlin AV ČR
https://schema.org/isbn	80-86778-16-9
http://localhost/t...ganizacniJednotka	14330

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