About: In vivo imaging of cell interior using automated high-resolution cytometry

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The fluorescent proteins are important innovation in the field of cell biology and in vivo experiments that use fluorescent proteins bring new interesting results from a different point of view than in vitro and in situ experiments. This presentation will introduce our automated 2D/3D high-resolution time-lapse image acquisition and analysis of living cells transfected with plasmids encoding fusion proteins or dyed with fluorescence probes. One of our in vivo projects, we will mention, is focused on the study of mitochondrial and nuclear apoptotic processes in living cells. Another in vivo project concentrates on the study of topography of telomeres and incidence of telomere-association phenomenon in various types of human tumor and healthy cells. In our experiments, cells are stably or transiently transfected with plasmid DNAs coding fusion mitochondrial apoptogenic or telomere-binding proteins with fluorescent proteins. The fluorescent proteins are important innovation in the field of cell biology and in vivo experiments that use fluorescent proteins bring new interesting results from a different point of view than in vitro and in situ experiments. This presentation will introduce our automated 2D/3D high-resolution time-lapse image acquisition and analysis of living cells transfected with plasmids encoding fusion proteins or dyed with fluorescence probes. One of our in vivo projects, we will mention, is focused on the study of mitochondrial and nuclear apoptotic processes in living cells. Another in vivo project concentrates on the study of topography of telomeres and incidence of telomere-association phenomenon in various types of human tumor and healthy cells. In our experiments, cells are stably or transiently transfected with plasmid DNAs coding fusion mitochondrial apoptogenic or telomere-binding proteins with fluorescent proteins. (en)
Title	In vivo imaging of cell interior using automated high-resolution cytometry In vivo imaging of cell interior using automated high-resolution cytometry (en)
skos:prefLabel	In vivo imaging of cell interior using automated high-resolution cytometry In vivo imaging of cell interior using automated high-resolution cytometry (en)
skos:notation	RIV/00216224:14330/04:00009707!RIV11-AV0-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/04/0907), P(GP204/03/D031), P(IAA1065203), Z(AV0Z5004920), Z(MSM 143300002)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Amrichová, Jana Vařecha, Miroslav Kozubek, Michal Kozubek, Stanislav Matula, Pavel Matula, Petr
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	567634
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/04:00009707
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	living cell (en)
http://linked.open.../riv/klicoveSlovo	living cell
http://linked.open...ontrolniKodProRIV	[855485102656]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	Biophysics of the Genome
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	High-resolution cytomery of living cells Apoptosis-inducing factor (AIF): its translocation from mitochondria and its action on nuclear chromatin The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Kozubek, Stanislav Lukášová, Emilie Matula, Pavel Ondřej, Vladan Kozubek, Michal Matula, Petr Vařecha, Miroslav Amrichová, Jana
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2004-10-12 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143300002
number of pages	7 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Masaryk University
https://schema.org/isbn	80-210-3560-9
http://localhost/t...ganizacniJednotka	14330

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