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  • Laboratory of Optical Microscopy has been working on the automation of image analysis in fluorescence microscopy since mid-1990s. At that time mostly 2D image analysis of FISH stained cell nuclei was explored. The images contained isolated cells (such as blood cells) that did not form many clusters and, therefore, image segmentation (the most difficult part of image analysis) was quite easy to automate. Later on 3D images of FISH (or immunofluorescence) stained cell nuclei acquired in confocal mode were dealt with. Also in this case the cell nuclei were well separated from each other, however somewhat blurred in axial direction due to the inferior resolution along z-axis. Also for this case automated methods have been developed including algorithms for the computation of a mathematical model of cell (or cell nucleus) boundary in 3D. At the end of 1990s the necessity of tissue image analysis arose.
  • Laboratory of Optical Microscopy has been working on the automation of image analysis in fluorescence microscopy since mid-1990s. At that time mostly 2D image analysis of FISH stained cell nuclei was explored. The images contained isolated cells (such as blood cells) that did not form many clusters and, therefore, image segmentation (the most difficult part of image analysis) was quite easy to automate. Later on 3D images of FISH (or immunofluorescence) stained cell nuclei acquired in confocal mode were dealt with. Also in this case the cell nuclei were well separated from each other, however somewhat blurred in axial direction due to the inferior resolution along z-axis. Also for this case automated methods have been developed including algorithms for the computation of a mathematical model of cell (or cell nucleus) boundary in 3D. At the end of 1990s the necessity of tissue image analysis arose. (en)
Title
  • Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images
  • Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images (en)
skos:prefLabel
  • Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images
  • Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images (en)
skos:notation
  • RIV/00216224:14330/04:00009701!RIV11-AV0-14330___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA202/02/0804), P(GA202/04/0907), P(IAA5004306), P(IBS5004010), P(NC6987), Z(MSM 143300002)
http://linked.open...vai/riv/dodaniDat
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  • 555651
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14330/04:00009701
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Image Analysis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [37B814D43D91]
http://linked.open...v/mistoKonaniAkce
  • Brno
http://linked.open...i/riv/mistoVydani
  • Brno
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  • Biophysics of the Genome
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Matula, Pavel
  • Svoboda, David
  • Hubený, Jan
  • Kozubek, Michal
  • Matula, Petr
  • Krontorád, Petr
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
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  • Masaryk University
https://schema.org/isbn
  • 80-210-3560-9
http://localhost/t...ganizacniJednotka
  • 14330
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