About: Rapid Image Acquisition of Living Cells and its Limits

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Technique of high-resolution cytometry (HRCM) developed in our laboratory is capable of automated (2D as well as 3D) acquisition and analysis of fluorescent stained cells. The cytometer can process large number of cells with high resolution. The acquisition can be repeated in time and that enables live cell studies. If very short events in living cells are studied then the sampling frequency must be high. The highest sampling frequency depends on the technical parameters of the motorized parts of cytometer (especially on the camera frame rate, the speed of filter exchange and the speed of axial movement) and on the control software, which must be appropriately optimized. The limits of the current setup are discussed and available sampling frequencies for different acquisition modes are listed. Technique of high-resolution cytometry (HRCM) developed in our laboratory is capable of automated (2D as well as 3D) acquisition and analysis of fluorescent stained cells. The cytometer can process large number of cells with high resolution. The acquisition can be repeated in time and that enables live cell studies. If very short events in living cells are studied then the sampling frequency must be high. The highest sampling frequency depends on the technical parameters of the motorized parts of cytometer (especially on the camera frame rate, the speed of filter exchange and the speed of axial movement) and on the control software, which must be appropriately optimized. The limits of the current setup are discussed and available sampling frequencies for different acquisition modes are listed. (en)
Title	Rapid Image Acquisition of Living Cells and its Limits Rapid Image Acquisition of Living Cells and its Limits (en)
skos:prefLabel	Rapid Image Acquisition of Living Cells and its Limits Rapid Image Acquisition of Living Cells and its Limits (en)
skos:notation	RIV/00216224:14330/04:00009696!RIV11-AV0-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/04/0907), P(GP204/03/D034), P(IAA5004306), P(IBS5004010), Z(AV0Z5004920), Z(MSM 143300002)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Kozubek, Michal Kozubek, Stanislav Matula, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	583553
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/04:00009696
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Image analysis (en)
http://linked.open.../riv/klicoveSlovo	Image analysis
http://linked.open...ontrolniKodProRIV	[3D55297616B4]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno, Czech Republic
http://linked.open...i/riv/nazevZdroje	Biophysics of the Genome
http://linked.open...in/vavai/riv/obor	JD
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	High-resolution cytomery of living cells Threedimensional analysis of cell nuclei using image cytometry Human genome structure Development of new diagnostic techniques for oncology
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Kozubek, Stanislav Matula, Pavel Ondřej, Vladan Kozubek, Michal
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2004-10-12 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143300002
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Masaryk University
https://schema.org/isbn	80-210-3560-9
http://localhost/t...ganizacniJednotka	14330

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