About: Architecture of chromosomal territories in apoptotic cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL In comparison with the control, an increased number of centromeric FISH signals were observed in prolonged confluence treated K-562 cells induced to apoptosis. The nuclear architecture of selected chromosomes in apoptotic nuclei of human leukemic cells K-562 and HL-60 was investigated in the presented paper. Etoposide and prolonged confluence were used for the induction of apoptosis. DAPI- as well as TUNEL- labelling of apoptotic nuclear bodies was combined with visualisation of chromosomal territories by the FISH technique. Simultaneous vital staining by Annexin V, PI and Hoechst 33342 was applied in order to distinguish apoptotic, necrotic and intact cell fraction of tested population. Our FISH analyses revealed that the 3D- structure of apoptotic nuclei as well as the 3D- structure of apoptotic bodies is preserved in formaldehyde fixed cells. High molecular weight DNA fragmentation was determined in apoptotic K-562 cells in contrast to oligonucleosomal cleavage observed in apoptotic HL In comparison with the control, an increased number of centromeric FISH signals were observed in prolonged confluence treated K-562 cells induced to apoptosis. (en)
Title	Architecture of chromosomal territories in apoptotic cells Architecture of chromosomal territories in apoptotic cells (en)
skos:prefLabel	Architecture of chromosomal territories in apoptotic cells Architecture of chromosomal territories in apoptotic cells (en)
skos:notation	RIV/00216224:14330/03:00008243!RIV11-AV0-14330___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/01/0186), P(IAB5004102), P(IBS5004010), Z(MSM 143300002)
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Bártová, Eva Kozubek, Stanislav Matula, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	599033
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/03:00008243
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	human genome structure (en)
http://linked.open.../riv/klicoveSlovo	human genome structure
http://linked.open...ontrolniKodProRIV	[63F5EC957019]
http://linked.open...v/mistoKonaniAkce	Hlohovec,Czech Republic
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	Biophysics of the Genome
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Investigation of the local control of gene expression using spectral microscopy and omage analysis techniques Nuclear topology of some protooncogenes in human neutrophilic granulocytes and leukemic cells Development of new diagnostic techniques for oncology
http://linked.open...UplatneniVysledku	2003
http://linked.open...iv/tvurceVysledku	Bártová, Eva Kozubek, Stanislav Matula, Pavel Souček, Karel Fojtová, Miloslava Palša, Stanislav
http://linked.open...vavai/riv/typAkce	EUR - Evropská
http://linked.open.../riv/zahajeniAkce	2003-01-01 (xsd:date)
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143300002
number of pages	10 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Masaryk University
https://schema.org/isbn	80-210-3226-X
http://localhost/t...ganizacniJednotka	14330

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