About: Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The mechanism of fatty acid-dependent uncoupling by mitochondrial uncoupling proteins (UCP) is still in debate. We have hypothesized that the anionic fatty acid head group is translocated by UCP, and the proton is transported electroneutrally in the bilayer by flip-flop of the protonated fatty acid. Alkylsulfonates are useful as probes of the UCP transport mechanism. They are analogues of fatty acids, and they are transported by UCP1, UCP2, and UCP3. We show that undecanesulfonate and laurate are mutually competitive inhibitors, supporting the hypothesis that fatty acid anion is transported by UCP1. Alkylsulfonates cannot be protonated because of their low pK(a), consequently, they cannot catalyze electroneutral proton transport in the bilayer and cannot support uncoupling by UCP. We report for the first time that propranolol forms permeant ion pairs with the alkylsulfonates, thereby removing this restriction. The mechanism of fatty acid-dependent uncoupling by mitochondrial uncoupling proteins (UCP) is still in debate. We have hypothesized that the anionic fatty acid head group is translocated by UCP, and the proton is transported electroneutrally in the bilayer by flip-flop of the protonated fatty acid. Alkylsulfonates are useful as probes of the UCP transport mechanism. They are analogues of fatty acids, and they are transported by UCP1, UCP2, and UCP3. We show that undecanesulfonate and laurate are mutually competitive inhibitors, supporting the hypothesis that fatty acid anion is transported by UCP1. Alkylsulfonates cannot be protonated because of their low pK(a), consequently, they cannot catalyze electroneutral proton transport in the bilayer and cannot support uncoupling by UCP. We report for the first time that propranolol forms permeant ion pairs with the alkylsulfonates, thereby removing this restriction. (en)
Title	Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling (en)
skos:prefLabel	Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling Alkylsulfonates as probes of uncoupling protein transport mechanism: ion pair transport demonstrates that H+ translocation by UCP1 is not necessary for uncoupling (en)
skos:notation	RIV/00216224:14330/01:00030508!RIV10-MSM-14330___
http://linked.open...avai/riv/aktivita	V
http://linked.open...avai/riv/aktivity	V
http://linked.open...iv/cisloPeriodika	34
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Vařecha, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	672867
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/01:00030508
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	UCP1; uncoupling protein; mitochondria (en)
http://linked.open.../riv/klicoveSlovo	UCP1 uncoupling protein mitochondria
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[1AC23154A32B]
http://linked.open...i/riv/nazevZdroje	Journal of Biological Chemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	276
http://linked.open...iv/tvurceVysledku	Vařecha, Miroslav Ježek, P. Garlid, K. D. Jabůrek, M.
http://linked.open...ain/vavai/riv/wos	000170613500060
issn	0021-9258
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 67 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software