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Description
  • We have analysed the chromatin structure of two subtelomeric tandem repeats from the plant Silene latifolia: X43.1 and 15Ssp. X43.1 shows two distinct nucleosome periodicities - 157 and 188bp. Preferred positions of its two nucleosomes have been mapped at both low and high resolution and the experimental results correspond to computer-redicted positions. 15Ssp is a newly-discovered sequence showing a telomere-associated position by PCR and a subtelomeric location by pulsed-field gel electrophoresis and fluorescence in situ hybridisation. Its 159 bp sequence unit shows a tandem arrangement and the presence of micrococcal nuclease-hypersensitive sites. Use of a chemical nuclease results in a regular nucleosome ladder of 157 bp periodicity. 15Ssp mononucleosomes show instability and absence of specific positioning, features typical for telomeric chromatin.
  • We have analysed the chromatin structure of two subtelomeric tandem repeats from the plant Silene latifolia: X43.1 and 15Ssp. X43.1 shows two distinct nucleosome periodicities - 157 and 188bp. Preferred positions of its two nucleosomes have been mapped at both low and high resolution and the experimental results correspond to computer-redicted positions. 15Ssp is a newly-discovered sequence showing a telomere-associated position by PCR and a subtelomeric location by pulsed-field gel electrophoresis and fluorescence in situ hybridisation. Its 159 bp sequence unit shows a tandem arrangement and the presence of micrococcal nuclease-hypersensitive sites. Use of a chemical nuclease results in a regular nucleosome ladder of 157 bp periodicity. 15Ssp mononucleosomes show instability and absence of specific positioning, features typical for telomeric chromatin. (en)
  • We have analysed the chromatin structure of two subtelomeric tandem repeats from the plant Silene latifolia: X43.1 and 15Ssp. X43.1 shows two distinct nucleosome periodicities - 157 and 188bp. Preferred positions of its two nucleosomes have been mapped at both low and high resolution and the experimental results correspond to computer-redicted positions. 15Ssp is a newly-discovered sequence showing a telomere-associated position by PCR and a subtelomeric location by pulsed-field gel electrophoresis and fluorescence in situ hybridisation. Its 159 bp sequence unit shows a tandem arrangement and the presence of micrococcal nuclease-hypersensitive sites. Use of a chemical nuclease results in a regular nucleosome ladder of 157 bp periodicity. 15Ssp mononucleosomes show instability and absence of specific positioning, features typical for telomeric chromatin. (cs)
Title
  • Transition between two forms of heterochromatin at plant subtelomeres
  • Transition between two forms of heterochromatin at plant subtelomeres (en)
  • Transition between two forms of heterochromatin at plant subtelomeres (cs)
skos:prefLabel
  • Transition between two forms of heterochromatin at plant subtelomeres
  • Transition between two forms of heterochromatin at plant subtelomeres (en)
  • Transition between two forms of heterochromatin at plant subtelomeres (cs)
skos:notation
  • RIV/00216224:14330/01:00004757!RIV08-AV0-14330___
http://linked.open.../vavai/riv/strany
  • 309
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(KSK5011112), P(VS97032), Z(AV0Z5004920), Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika
  • January
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 699058
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14330/01:00004757
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • chromatin structure; nucleosome positioning; plant telomeres and subtelomeres (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [89C3AF4B249E]
http://linked.open...i/riv/nazevZdroje
  • Chromosome Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • roč. 9
http://linked.open...iv/tvurceVysledku
  • Sýkorová, Eva
  • Fajkus, Jiří
  • Fukui, Kiichi
  • Mikako, Ito
http://linked.open...n/vavai/riv/zamer
issn
  • 0967-3849
number of pages
http://localhost/t...ganizacniJednotka
  • 14330
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