About: Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana.     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • Cytokinins are plant hormones involved in regulation of a number of responses in plants. Deciphering molecular mechanisms of cytokinin perception and signal transduction is a crucial step in analysis of molecular mechanism of cytokonin action. Recently, a putative cytokinin receptor, CKI1, has been identified in Arabidopsis thaliana by activation tagging. Sequence analysis revealed that CKI1 belongs to a family of sensor hybrid histidine kinases. We have subcloned individual CKI1 domains and investigated feasibility of their production in a bacterial expression system. Here we report a succesful production and single-step purification of the CKI1 signal receiver domain. The open reading frame coding for the domain was cloned into an expression vector pET-28 in a way leading to a fusion protein with two hexahistidine tags. The resulting fusion protein was produced in E. coli. Single-step purification of the fusion protein was achieved by immobilized metal affinity chromatography. Development of the pur
  • Cytokinins are plant hormones involved in regulation of a number of responses in plants. Deciphering molecular mechanisms of cytokinin perception and signal transduction is a crucial step in analysis of molecular mechanism of cytokonin action. Recently, a putative cytokinin receptor, CKI1, has been identified in Arabidopsis thaliana by activation tagging. Sequence analysis revealed that CKI1 belongs to a family of sensor hybrid histidine kinases. We have subcloned individual CKI1 domains and investigated feasibility of their production in a bacterial expression system. Here we report a succesful production and single-step purification of the CKI1 signal receiver domain. The open reading frame coding for the domain was cloned into an expression vector pET-28 in a way leading to a fusion protein with two hexahistidine tags. The resulting fusion protein was produced in E. coli. Single-step purification of the fusion protein was achieved by immobilized metal affinity chromatography. Development of the pur (en)
Title
  • Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana.
  • Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana. (en)
skos:prefLabel
  • Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana.
  • Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana. (en)
skos:notation
  • RIV/00216224:14330/01:00004630!RIV/2002/MSM/143302/N
http://linked.open.../vavai/riv/strany
  • 30
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LN00A081), P(VS96096)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 680068
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14330/01:00004630
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [3B1288C9EFD6]
http://linked.open...v/mistoKonaniAkce
  • Brno
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • V. Pracovní setkání biochemiků a molekulárních biologů, Sborník příspěvků, Brno 2001
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...ocetUcastnikuAkce
http://linked.open...nichUcastnikuAkce
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Hejátko, Jan
  • Zouhar, Jan
  • Brzobohatý, Břetislav
  • Borkovcová, Petra
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Masarykova univerzita v Brně
https://schema.org/isbn
  • 80-210-2538-7
http://localhost/t...ganizacniJednotka
  • 14330
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software