Attributes | Values |
---|
rdf:type
| |
Description
| - Cytokinins are plant hormones involved in regulation of a number of responses in plants. Deciphering molecular mechanisms of cytokinin perception and signal transduction is a crucial step in analysis of molecular mechanism of cytokonin action. Recently, a putative cytokinin receptor, CKI1, has been identified in Arabidopsis thaliana by activation tagging. Sequence analysis revealed that CKI1 belongs to a family of sensor hybrid histidine kinases. We have subcloned individual CKI1 domains and investigated feasibility of their production in a bacterial expression system. Here we report a succesful production and single-step purification of the CKI1 signal receiver domain. The open reading frame coding for the domain was cloned into an expression vector pET-28 in a way leading to a fusion protein with two hexahistidine tags. The resulting fusion protein was produced in E. coli. Single-step purification of the fusion protein was achieved by immobilized metal affinity chromatography. Development of the pur
- Cytokinins are plant hormones involved in regulation of a number of responses in plants. Deciphering molecular mechanisms of cytokinin perception and signal transduction is a crucial step in analysis of molecular mechanism of cytokonin action. Recently, a putative cytokinin receptor, CKI1, has been identified in Arabidopsis thaliana by activation tagging. Sequence analysis revealed that CKI1 belongs to a family of sensor hybrid histidine kinases. We have subcloned individual CKI1 domains and investigated feasibility of their production in a bacterial expression system. Here we report a succesful production and single-step purification of the CKI1 signal receiver domain. The open reading frame coding for the domain was cloned into an expression vector pET-28 in a way leading to a fusion protein with two hexahistidine tags. The resulting fusion protein was produced in E. coli. Single-step purification of the fusion protein was achieved by immobilized metal affinity chromatography. Development of the pur (en)
|
Title
| - Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana.
- Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana. (en)
|
skos:prefLabel
| - Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana.
- Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana. (en)
|
skos:notation
| - RIV/00216224:14330/01:00004630!RIV/2002/MSM/143302/N
|
http://linked.open.../vavai/riv/strany
| |
http://linked.open...avai/riv/aktivita
| |
http://linked.open...avai/riv/aktivity
| |
http://linked.open...vai/riv/dodaniDat
| |
http://linked.open...aciTvurceVysledku
| |
http://linked.open.../riv/druhVysledku
| |
http://linked.open...iv/duvernostUdaju
| |
http://linked.open...titaPredkladatele
| |
http://linked.open...dnocenehoVysledku
| |
http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14330/01:00004630
|
http://linked.open...riv/jazykVysledku
| |
http://linked.open.../riv/klicovaSlova
| - Expression and Single-Step Purification of the Signal Receiver Domain of CKI1, a Putative Cytokinin Receptor from Arabidopsis Thaliana. (en)
|
http://linked.open.../riv/klicoveSlovo
| |
http://linked.open...ontrolniKodProRIV
| |
http://linked.open...v/mistoKonaniAkce
| |
http://linked.open...i/riv/mistoVydani
| |
http://linked.open...i/riv/nazevZdroje
| - V. Pracovní setkání biochemiků a molekulárních biologů, Sborník příspěvků, Brno 2001
|
http://linked.open...in/vavai/riv/obor
| |
http://linked.open...ichTvurcuVysledku
| |
http://linked.open...cetTvurcuVysledku
| |
http://linked.open...ocetUcastnikuAkce
| |
http://linked.open...nichUcastnikuAkce
| |
http://linked.open...vavai/riv/projekt
| |
http://linked.open...UplatneniVysledku
| |
http://linked.open...iv/tvurceVysledku
| - Hejátko, Jan
- Zouhar, Jan
- Brzobohatý, Břetislav
- Borkovcová, Petra
|
http://linked.open...vavai/riv/typAkce
| |
http://linked.open.../riv/zahajeniAkce
| |
number of pages
| |
http://purl.org/ne...btex#hasPublisher
| - Masarykova univerzita v Brně
|
https://schema.org/isbn
| |
http://localhost/t...ganizacniJednotka
| |