About: Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A novel system allowing a non-invasive manipulation of cytokinin levels is being developed in A. thaliana. Transcriptional fusions between isopentenyl transferase gene, ipt, and a reporter gene, GUS, were inserted behind a synthetic promoter (pOp). pOp is expected to be inactive when introduced into wild type plants but is activated when these plants are crossed with others that express a novel transcription activation protein, LhG4. Transgenic Arabidopsis plants carrying pOp-ipt-GUS and pOp-GUS-ipt were generated by Agrobacterium infiltration technique. The transformed plants developed without any detectable phenotype alteration indicating that pOp promoter is virtually silent in the transformants in the absence of LhG4. The first evidence for activation of ipt and GUS in pOp-ipt-GUS and pOp-GUS-ipt was provided by crossing the individual transformants with an activator line carrying LhG4 under control of CaMV35S promoter (CaMV35S-LhG4). We will report phenotype alterations observed in progeny of cro A novel system allowing a non-invasive manipulation of cytokinin levels is being developed in A. thaliana. Transcriptional fusions between isopentenyl transferase gene, ipt, and a reporter gene, GUS, were inserted behind a synthetic promoter (pOp). pOp is expected to be inactive when introduced into wild type plants but is activated when these plants are crossed with others that express a novel transcription activation protein, LhG4. Transgenic Arabidopsis plants carrying pOp-ipt-GUS and pOp-GUS-ipt were generated by Agrobacterium infiltration technique. The transformed plants developed without any detectable phenotype alteration indicating that pOp promoter is virtually silent in the transformants in the absence of LhG4. The first evidence for activation of ipt and GUS in pOp-ipt-GUS and pOp-GUS-ipt was provided by crossing the individual transformants with an activator line carrying LhG4 under control of CaMV35S promoter (CaMV35S-LhG4). We will report phenotype alterations observed in progeny of cro (en)
Title	Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development. Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development. (en)
skos:prefLabel	Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development. Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development. (en)
skos:notation	RIV/00216224:14330/01:00004568!RIV/2002/AV0/143302/N
http://linked.open.../vavai/riv/strany	88
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(IAA5004001)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Jian Chun, Guo Brzobohatý, Břetislav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	694346
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/01:00004568
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development. (en)
http://linked.open.../riv/klicoveSlovo	Regulated Expression of Isopentenyl Transferase in Arabidopsis: System Development.
http://linked.open...ontrolniKodProRIV	[8989D4D88A39]
http://linked.open...v/mistoKonaniAkce	Brno
http://linked.open...i/riv/mistoVydani	Brno
http://linked.open...i/riv/nazevZdroje	17th International Conference on Plant Growth Substances, Brno, Czech Republic, July 1-6, 2001, Abstracts
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Transkription activation system for studying the relationship between cytokinin metabolism and action in Arabidopsis and tobacco
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Brzobohatý, Břetislav Jian Chun, Guo
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2001-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Mendelova zemědělská a lesnická univerzita v Brně
https://schema.org/isbn	80-7157-518-6
http://localhost/t...ganizacniJednotka	14330

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