About: Theoretical versus experimental resolution in optical microscopy

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The aim of this article is to compare experimental resolution under different conditions with theoretical resolution predicted using electromagnetic diffraction theory. Imaging properties of fluorescent beads of three different diameters (0.1 mum, 0.2 mum, and 0.5 mum) as well as imaging properties of four different fluorescence-stained DNA targets (ABL gene, BCR gene, centromere 6, and centromere 17) are studied. It is shown how the dependence of the resolution on object size varies with wavelength (520 nm versus 580 nm), type of microscopy (wide-field, confocal using Nipkow disk, confocal laser scanning) and basic image processing steps (median and gaussian filters). Furthermore, specimen influence on the resolution was studied (the influence of embedding medium, coverglass thickness, and depth below the coverglass). Both lateral and axial resolutions are presented. The results clearly show that real objects are far from being points and that experimental resolution is often much worse than the theo The aim of this article is to compare experimental resolution under different conditions with theoretical resolution predicted using electromagnetic diffraction theory. Imaging properties of fluorescent beads of three different diameters (0.1 mum, 0.2 mum, and 0.5 mum) as well as imaging properties of four different fluorescence-stained DNA targets (ABL gene, BCR gene, centromere 6, and centromere 17) are studied. It is shown how the dependence of the resolution on object size varies with wavelength (520 nm versus 580 nm), type of microscopy (wide-field, confocal using Nipkow disk, confocal laser scanning) and basic image processing steps (median and gaussian filters). Furthermore, specimen influence on the resolution was studied (the influence of embedding medium, coverglass thickness, and depth below the coverglass). Both lateral and axial resolutions are presented. The results clearly show that real objects are far from being points and that experimental resolution is often much worse than the theo (en)
Title	Theoretical versus experimental resolution in optical microscopy Theoretical versus experimental resolution in optical microscopy (en)
skos:prefLabel	Theoretical versus experimental resolution in optical microscopy Theoretical versus experimental resolution in optical microscopy (en)
skos:notation	RIV/00216224:14330/01:00004204!RIV/2002/GA0/143302/N
http://linked.open.../vavai/riv/strany	157
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/99/P008), P(NM15), P(VS97031), Z(MSM 143300002)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Kozubek, Michal
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Fakulta informatiky
http://linked.open...dnocenehoVysledku	698681
http://linked.open...ai/riv/idVysledku	RIV/00216224:14330/01:00004204
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	resolution, confocal microscopy, wide-field microscopy, image processing, fluorescent bead (en)
http://linked.open.../riv/klicoveSlovo	image processing confocal microscopy resolution wide-field microscopy fluorescent bead
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A23E594DBE3E]
http://linked.open...i/riv/nazevZdroje	Microscopy research and technique
http://linked.open...in/vavai/riv/obor	JD
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Image analysis in the study of interphase nucleus structure The use interphase FISH for the determination of individuals with elevated risk of the induction of hemoblastoses Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases
http://linked.open...UplatneniVysledku	2001
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Kozubek, Michal
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143300002
issn	1059-910X
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14330

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