About: Chromatographic separation of lambda DNas

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The separation of DNA fragments are of increasing interest of molecular biology, gene technology and applied sciences. The most widely used methods, the polyacrylamide and agarose gel electrophoresis, have been limited to comparatively small molecules. Moreover the fragments are contamined by soluble impurities from gels, which are known to be inhibitors of DNA processing enzymes. The separation of DNA fragments larger than 20 kbp, which are important as inserts in recombinant DNA research, has been studied. An alternative method based on gel permeation chromatography (GPC) has been adopted for this purpose. In our work GPC on HEMA based sorbents (copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate) was used for separation of wild type lambda DNA molecules (48.5 kbp), their concatemers and mixture of lambda DNA fragments. Three peaks representing the 9.4, 23.1 and 48.5 kbp were eluted in the order of the slalom mode. The separation of lambda DNA concatemers was not achieved. The separation of DNA fragments are of increasing interest of molecular biology, gene technology and applied sciences. The most widely used methods, the polyacrylamide and agarose gel electrophoresis, have been limited to comparatively small molecules. Moreover the fragments are contamined by soluble impurities from gels, which are known to be inhibitors of DNA processing enzymes. The separation of DNA fragments larger than 20 kbp, which are important as inserts in recombinant DNA research, has been studied. An alternative method based on gel permeation chromatography (GPC) has been adopted for this purpose. In our work GPC on HEMA based sorbents (copolymer of 2-hydroxyethyl methacrylate and ethylene dimethacrylate) was used for separation of wild type lambda DNA molecules (48.5 kbp), their concatemers and mixture of lambda DNA fragments. Three peaks representing the 9.4, 23.1 and 48.5 kbp were eluted in the order of the slalom mode. The separation of lambda DNA concatemers was not achieved. (en)
Title	Chromatographic separation of lambda DNas Chromatographic separation of lambda DNas (en)
skos:prefLabel	Chromatographic separation of lambda DNas Chromatographic separation of lambda DNas (en)
skos:notation	RIV/00216224:14310/99:00004113!RIV/2002/GA0/143102/N
http://linked.open.../vavai/riv/strany	141
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA203/98/1231)
http://linked.open...vai/riv/dodaniDat	2002
http://linked.open...aciTvurceVysledku	Racek, Tomáš Španová, Alena Doškař, Jiří Rittich, Bohuslav
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	736886
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/99:00004113
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chromatographic separation of lambda DNas (en)
http://linked.open.../riv/klicoveSlovo	Chromatographic separation of lambda DNas
http://linked.open...ontrolniKodProRIV	[2234E6B7385C]
http://linked.open...v/mistoKonaniAkce	Bratislava
http://linked.open...i/riv/mistoVydani	Bratislava
http://linked.open...i/riv/nazevZdroje	13th Bratislava International Conference on Polymers. Separation and Characterization of Macromolecules
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Oriented immobilization of enzymes and antibodies using biospecific interactions
http://linked.open...UplatneniVysledku	1999
http://linked.open...iv/tvurceVysledku	Doškař, Jiří Rittich, Bohuslav Španová, Alena Racek, Tomáš
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	1999-01-01 (xsd:date)
number of pages	1 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Polymer Institute of the Slovak Academy of Sciences
http://localhost/t...ganizacniJednotka	14310

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