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Description
| - Chromatography is an integral part of large scale purification of plasmids. Samples usualy contains, except to cell components obtained after cell lysis, chromosomal DNA fragments, high molecular-weight RNA and plasmid variants. To achieve good chromatographic resolution it is necessary to degrade RNA by RNase. The aim of our work was to utilize RNase A immobilized on magnetic bead cellulose for degradation of high molecular-weight RNA before chromatographic purification of plasmid DNA. The enzyme was coupled to the particles via cyanuric chloride method. Temperature stability of soluble and immobilized RNase A was estimated after preincubation of the samples at different temperatures. Comparison of the pattern of soluble and immobilized RNase A showed a pH shift in activity. Practical use of magnetic particles in the purification of plasmid DNA is demonstrated.
- Chromatography is an integral part of large scale purification of plasmids. Samples usualy contains, except to cell components obtained after cell lysis, chromosomal DNA fragments, high molecular-weight RNA and plasmid variants. To achieve good chromatographic resolution it is necessary to degrade RNA by RNase. The aim of our work was to utilize RNase A immobilized on magnetic bead cellulose for degradation of high molecular-weight RNA before chromatographic purification of plasmid DNA. The enzyme was coupled to the particles via cyanuric chloride method. Temperature stability of soluble and immobilized RNase A was estimated after preincubation of the samples at different temperatures. Comparison of the pattern of soluble and immobilized RNase A showed a pH shift in activity. Practical use of magnetic particles in the purification of plasmid DNA is demonstrated. (en)
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Title
| - Characterisation of ribonuclease immobilised on cellulose and HEMA supports
- Characterisation of ribonuclease immobilised on cellulose and HEMA supports (en)
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skos:prefLabel
| - Characterisation of ribonuclease immobilised on cellulose and HEMA supports
- Characterisation of ribonuclease immobilised on cellulose and HEMA supports (en)
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skos:notation
| - RIV/00216224:14310/99:00003568!RIV/2002/GA0/143102/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14310/99:00003568
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Characterisation of ribonuclease immobilised on cellulose and HEMA supports (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...v/mistoKonaniAkce
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http://linked.open...i/riv/mistoVydani
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http://linked.open...i/riv/nazevZdroje
| - 13th International Symposium of Affinity Technology and Bio-Recognition
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...ocetUcastnikuAkce
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http://linked.open...nichUcastnikuAkce
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Horák, Daniel
- Rittich, Bohuslav
- Šafář, Jan
- Španová, Alena
- Lenfeld, Jiří
- Beneš, Milan
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http://linked.open...vavai/riv/typAkce
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http://linked.open.../riv/zahajeniAkce
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Université de Technologie de Compiegne
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http://localhost/t...ganizacniJednotka
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