About: A cytospin technique for spreading plant metaphases suitable for immunofluorescence studies.

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About: A cytospin technique for spreading plant metaphases suitable for immunofluorescence studies. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Recent immunofluorescence techniques enable the localization of various cellular antigens, thus providing a powerful tool for cell and molecular biology research. Serious problems occur, however, when these techniques are applied to plant material. The presence of the cellulose wall can be a barrier to reproducible penetration of antibodies into cells and it often displays a confusing autofluorescence. A novel technique to prepare mitotic chromosome spreads from root tip meristems of germinating seeds is presented. Synchronous mitotic cells arrested in metaphase are converted into protoplasts using pectin and cellulose hydrolytic enzymes, and the purified protoplasts are fixed either in a methanol-acetic acid mixture to study DNA epitopes or in a nonextracting fixative to study chromosomal proteins. The latter fixative contains Triton X-100 to lyse the protoplasts and neutral formaldehyde to fix proteins by cross-linking. The protoplasts are immediately centrifuged onto microscopic slides as commonly Recent immunofluorescence techniques enable the localization of various cellular antigens, thus providing a powerful tool for cell and molecular biology research. Serious problems occur, however, when these techniques are applied to plant material. The presence of the cellulose wall can be a barrier to reproducible penetration of antibodies into cells and it often displays a confusing autofluorescence. A novel technique to prepare mitotic chromosome spreads from root tip meristems of germinating seeds is presented. Synchronous mitotic cells arrested in metaphase are converted into protoplasts using pectin and cellulose hydrolytic enzymes, and the purified protoplasts are fixed either in a methanol-acetic acid mixture to study DNA epitopes or in a nonextracting fixative to study chromosomal proteins. The latter fixative contains Triton X-100 to lyse the protoplasts and neutral formaldehyde to fix proteins by cross-linking. The protoplasts are immediately centrifuged onto microscopic slides as commonly (en)
Title	A cytospin technique for spreading plant metaphases suitable for immunofluorescence studies. A cytospin technique for spreading plant metaphases suitable for immunofluorescence studies. (en)
skos:prefLabel	A cytospin technique for spreading plant metaphases suitable for immunofluorescence studies. A cytospin technique for spreading plant metaphases suitable for immunofluorescence studies. (en)
skos:notation	RIV/00216224:14310/98:00009350!RIV/2004/MSM/143104/N
http://linked.open.../vavai/riv/strany	150-156
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 143100008)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2004
http://linked.open...aciTvurceVysledku	Pitelová, Renáta Vyskot, Boris Široký, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	760142
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/98:00009350
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	immunofluorescence technique (en)
http://linked.open.../riv/klicoveSlovo	immunofluorescence technique
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[9B28DBC3707F]
http://linked.open...i/riv/nazevZdroje	Biotech Histochem
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	1998
http://linked.open...v/svazekPeriodika	73
http://linked.open...iv/tvurceVysledku	Vyskot, Boris Široký, Jiří Hladilová, Renáta
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20143100008
issn	1052-0295
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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