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  • Biogenic amines as biologically active molecules with potential toxicological dangers are often subject of interest in food analysis. Since these substances do not exhibit significant fluorescence nor absorbance, derivatization is necessary for analysis in combination with RP-HPLC-UV setup. The use of experimental design (ED) in combination with artificial neural networks (ANN) for optimization of dansyl derivatization conditions for seven biogenic amines is presented. Four key factors were studied – concentration of derivatization reagent, buffer pH, temperature and incubation time. With the use of ED-ANN approach, optimal conditions for dansyl chloride derivatization were found and experimentally confirmed.
  • Biogenic amines as biologically active molecules with potential toxicological dangers are often subject of interest in food analysis. Since these substances do not exhibit significant fluorescence nor absorbance, derivatization is necessary for analysis in combination with RP-HPLC-UV setup. The use of experimental design (ED) in combination with artificial neural networks (ANN) for optimization of dansyl derivatization conditions for seven biogenic amines is presented. Four key factors were studied – concentration of derivatization reagent, buffer pH, temperature and incubation time. With the use of ED-ANN approach, optimal conditions for dansyl chloride derivatization were found and experimentally confirmed. (en)
Title
  • Use of artificial neural networks for optimization of biogenic amines derivatization
  • Use of artificial neural networks for optimization of biogenic amines derivatization (en)
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  • Use of artificial neural networks for optimization of biogenic amines derivatization
  • Use of artificial neural networks for optimization of biogenic amines derivatization (en)
skos:notation
  • RIV/00216224:14310/14:00077326!RIV15-MSM-14310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED1.1.00/02.0068)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 52239
http://linked.open...ai/riv/idVysledku
  • RIV/00216224:14310/14:00077326
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • ANN; biogenic amines; dansyl chloride; HPLC (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [41841B524981]
http://linked.open...v/mistoKonaniAkce
  • Brno
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • CECE 2014
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Lubal, Přemysl
  • Farková, Marta
  • Havliš, Jan
  • Farka, Zdeněk
  • Rozman, Tomáš
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
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  • Ústav analytické chemie AV ČR
https://schema.org/isbn
  • 9788090495920
http://localhost/t...ganizacniJednotka
  • 14310
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