About: Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection     Goto   Sponge   NotDistinct   Permalink

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  • Amino acids are very important compounds in living organisms and their quantification in biological fluids can be used for diagnostic purposes. For determination of amino acids by capillary electrophoresis coupled with laser induced fluorescence detection, derivatization procedure is necessary because only a few amino acids exhibit native fluorescence. Derivatization reagent naphthalene-2,3-dicarboxaldehyde reacts with primary amines in the presence of nucleophile, such as cyanide anion, to form fluorescent derivates. Derivatization reaction can be done in pre-capillary or on-capillary arrangement. In on-capillary arrangement the capillary is used both for separation and derivatization. Optimization of pre-capillary and on-capillary derivatization procedure of amino acids using naphthalene-2,3-dicarboxaldehyde and NaCN, and comparison of these approaches is subject of this contribution.
  • Amino acids are very important compounds in living organisms and their quantification in biological fluids can be used for diagnostic purposes. For determination of amino acids by capillary electrophoresis coupled with laser induced fluorescence detection, derivatization procedure is necessary because only a few amino acids exhibit native fluorescence. Derivatization reagent naphthalene-2,3-dicarboxaldehyde reacts with primary amines in the presence of nucleophile, such as cyanide anion, to form fluorescent derivates. Derivatization reaction can be done in pre-capillary or on-capillary arrangement. In on-capillary arrangement the capillary is used both for separation and derivatization. Optimization of pre-capillary and on-capillary derivatization procedure of amino acids using naphthalene-2,3-dicarboxaldehyde and NaCN, and comparison of these approaches is subject of this contribution. (en)
Title
  • Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection
  • Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection (en)
skos:prefLabel
  • Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection
  • Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection (en)
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  • RIV/00216224:14310/14:00074190!RIV15-GA0-14310___
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  • P(GAP206/11/0009), P(GBP206/12/G014)
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  • 34940
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  • RIV/00216224:14310/14:00074190
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  • capillary electrophoresis; fluorescence detection; derivatization; amino acids (en)
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  • [36C43AEF5467]
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  • Brno
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  • Brno
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  • 11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014
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  • Glatz, Zdeněk
  • Celá, Andrea
  • Mádr, Aleš
  • Dědová, Tereza
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number of pages
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  • Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika
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  • 9788090495920
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  • 14310
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